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pACT1:Cas9-GFP, U6:sgUPRT

Plasmid

#122853

Purpose

Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

Cas9-GFP
U6-sgUPRT

Use

CRISPR; Cas9-gfp plasmid for genome editing of cr…

Tags

eGFP

Promoter

Cryptosporidium parvum U6 and Cryptosporidium par…

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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ECFP-IKB

Plasmid

#111193

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

IKB-alpha

Tags

ECFP

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B GG

Plasmid

#89451

Purpose

Expression of GFP-tagged human Rab27B GG in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B GG (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

GG (geranyl geranyl site is mutated)

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)

Plasmid

#70191

Purpose

Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

ClpX (clpX )

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pBdox-SPI1-T2A-CEBPA-P2A-FLI1_BleoR

Plasmid

#250291

Purpose

PiggyBac integration of 3 transcription factors (SPI1, CEBPA, FLI1) under a dox-inducible promoter

Depositor

George Church

Article

Liu et al Nat Commun. 2025 Jun 10;16(1):5136. doi:

Insert

SPI1-T2A-CEBPA-P2A-FLI1

Expression

Mammalian

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pET30-2-PHGDH

Plasmid

#83907

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pET-21a-PE2-His

Plasmid

#201973

Purpose

Express His-tagged PE2 in bacteria

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

PE2-HIS

Tags

His and c-myc NLS

Expression

Bacterial

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187951

Purpose

FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin

Plasmid

#187953

Purpose

FKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

FKBP12_F36V-SpdCas9-KRAB-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCX-SponGee-Kras

Plasmid

#134777

Purpose

mammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

SponGee-Kras

Tags

FLAG and mRFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Empty Target Control

Plasmid

#127667

Purpose

Encodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoter

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

CRY2-HA-2A-mCherry

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mKikGR1 (mKikGR1-V69T)

Plasmid

#99227

Purpose

Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mKikGR1

Expression

Bacterial

Mutation

monomeric variant with mutation V69T (Eos notatio…

Promoter

T7

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pUK21-NotI

Plasmid

#51660

Purpose

Bacterial cloning vector based on pUK21 (Kan)

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Cloning vector

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pUK21-deltaBB

Plasmid

#51658

Purpose

Bacterial cloning vector based on pUK21 (Kan)

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Cloning vector

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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PCx34.7-pcDNA

Plasmid

#42921

Depositor

John O'Brien

Article

O'Brien et al J Neurosci. 2004 Jun 16;24(24):5632-42.

Insert

Connexin 34.7

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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D-raf R217L

Plasmid

#39762

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Expression

Insect

Mutation

R217L

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
pcA17/F527

Plasmid

#17684

Depositor

David Shalloway

Article

Yaciuk et al Mol Cell Biol. 1989 Jun . 9(6):2453-63.

Insert

c-src S17A Y527F (SRC Chicken)

Expression

Mammalian

Mutation

Ser 17 changed to Ala. Tyr 527 changed to Phe .

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pAP699e

Plasmid

#253943

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Article

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Linker::T2A::Linker::GCamp6s::Linker+CAAX

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAP826e

Plasmid

#253946

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Article

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Begin-mNeonGreen::PH-domain::Linker::mCarmine::Linker::T2A::Linker

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAP311

Plasmid

#253947

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Article

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Linker::3xFlag::mVenus::HAtag::Linker

Available Since

May 20, 2026

Availability

Academic Institutions and Nonprofits only

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