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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,126 results for: LEA

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Showing: 4841 - 4860 of 12126 results

  1. O-LARS-15

    Plasmid
    #166542
    Purpose
    scFv of a human scaffold targeting Leucyl-tRNA synthetase. Antigen coverage aa 260-509 of 1176
    Depositor
    Susanne GrƤslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166542-plasmid-map-sequence-id-328271

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  2. pGBW-m4133269

    Plasmid
    #152855
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 ORF8 protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152855-plasmid-map-sequence-id-320320

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  3. pGBW-m4134143

    Plasmid
    #152022
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp2
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152022-plasmid-map-sequence-id-310621

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  4. QQR1 variant

    Plasmid
    #138564
    Purpose
    Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    QQR1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, Tā€¦
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138564-plasmid-map-sequence-id-274793

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  5. TBXTA-c026

    Plasmid
    #139763
    Purpose
    Protein expression/GST pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, Full-length, WT (TBXT Human)
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    Contains only amino acids S2- M435
    Promoter
    T7
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139763-plasmid-map-sequence-id-296576

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  6. TBXTA-c006

    Plasmid
    #139755
    Purpose
    For protein expression/crystallography (PDB:6F59)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-S224
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139755-plasmid-map-sequence-id-296509

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  9. pEPOR1CB0013

    Plasmid
    #117549
    Purpose
    Level 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
    Expression
    Plant
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available Since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117549-plasmid-map-sequence-id-228087

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  10. KLHL3 (KLHL3A-c003)

    Plasmid
    #110251
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KLHL3 (KLHL3 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110251-plasmid-map-sequence-id-212338

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  13. ZMYND8 (PRKCBP1A-c097)

    Plasmid
    #98244
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PRKCBP1A (ZMYND8 Human)
    Tags
    His6-Zb-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    98244-plasmid-map-sequence-id-193422

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  14. NUDT22 (NUDT22A-c001)

    Plasmid
    #98230
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT22A (NUDT22 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98230-plasmid-map-sequence-id-193410

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Showing: 4841 - 4860 of 12126 results