We narrowed to 34,532 results for: Sis

1. MSCV-TAP(FLAG-HA) CYLD

   Plasmid

   #60031

   Purpose

   CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Retroviral

   Tags

   FLAG, HA, and IRES Puro

   Expression

   Mammalian

   Mutation

   Promoter

   LTR-driven expression

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

2. CD8-dCherry-FRB

   Plasmid

   #100739

   Purpose

   To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N)

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CD8A (CD8A Human)
   

   Use

   Tags

   FRB and dCherry

   Expression

   Mammalian

   Mutation

   Dark mCherry (K70N) non-fluorescent

   Promoter

   CMV

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMMP-NGFR:LMP1-IRES-mRFP

   Plasmid

   #36974

   Depositor

   Bill Sugden

   Article

   Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

   Insert

   NGFR:LMP1 (NGFR, LMP-1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   NGFR:LMP1 chimeric receptor consists of aa 1–276 …

   Promoter

   CMV

   Available since

   Aug. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKIF1C-2xFLAG

   Plasmid

   #130979

   Purpose

   Expression of KIF1C-2xFLAG in mammalian cells.

   Depositor

   Anne Straube

   Article

   Theisen et al Dev Cell. 2012 Dec 11;23(6):1153-66. doi

   Insert

   KIF1C-2xFLAG (KIF1C Human)
   

   Use

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   4 silent mutations that make this construct resis…

   Promoter

   CMV

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FLEXfrt-SaCas9-U6-sgGria3

   Plasmid

   #124869

   Purpose

   Mutagenesis of Gria3

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria3 (Gria3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSUPER-retro-puro-tetO-Mek1-shRNA

   Plasmid

   #53179

   Purpose

   Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (Map2k1 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pAN19-3xFLAG-TIR1-NOSt

   Plasmid

   #108545

   Purpose

   Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

   Depositor

   Keiko Torii

   Article

   Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

   Insert

   TRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
   

   Use

   Cloning vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Gal4-VP16-HSF1Δ∼PRD

   Plasmid

   #71727

   Purpose

   plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1Δ∼PRD RD

   Depositor

   Lea Sistonen

   Article

   Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

   Insert

   Gal4 DBD HSF1 Δ∼PRD RD VP16 AD (HSF1 Budding Yeast, Herpes Simplex Virus protein VP16, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAN19-TIR1-GUS-NOSt

   Plasmid

   #108547

   Purpose

   Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

   Depositor

   Keiko Torii

   Article

   Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

   Insert

   TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
   

   Use

   Cloning vector

   Tags

   GUS

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAN19-ccvTIR1-GUS-NOSt

    Plasmid

    #108548

    Purpose

    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    GUS

    Expression

    Mutation

    Changed F79 to G

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-PIG-N3FLAG-TAF12

    Plasmid

    #105593

    Purpose

    retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    silent mutations to make it resistant to the targ…

    Promoter

    MSCV-LTR

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMCh-1522

    Plasmid

    #82428

    Purpose

    Plasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 CED 11W11A
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    amino acids 1-1158 of nvGW182 were deleted and Tr…

    Promoter

    EF1A

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMCh-1526

    Plasmid

    #82429

    Purpose

    Plasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 CED 11W11A
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    amino acids 1-1158 of nvGW182 were deleted and Tr…

    Promoter

    CMV

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWZLblasti-HA-MEK5 CBM-E

    Plasmid

    #53169

    Purpose

    Expresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK5 (MAP2K5 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L280A/H281A/M323A/Q332A

    Promoter

    Available since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

15. MP6

    Plasmid

    #69669

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA emrR ugi CDA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. MP1

    Plasmid

    #69627

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 umuD' umuC recA730
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MP-P

    Plasmid

    #69661

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA emrR mutH(E56A)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MP4

    Plasmid

    #69652

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pREDSIX

    Plasmid

    #74103

    Purpose

    mCherry-encoding vector for marker replacement mutagenesis in Bradyrhizobium diazoefficiens and other Alphaproteobacteria

    Depositor

    Hans-Martin Fischer

    Article

    Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

    Type

    Empty backbone

    Use

    Conjugative bacterial vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. MP5

    Plasmid

    #69677

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA ugi CDA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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