We narrowed to 19,636 results for: tec

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pBJ3

Plasmid

#167132

Purpose

A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

Depositor

Akira Mine

Article

Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

Insert

luxCDABE

Use

Luciferase

Expression

Bacterial

Promoter

Frr promoter

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAC1749

Plasmid

#127983

Purpose

AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

Cpf1

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

codon optimized for Aspergillus nidulans

Promoter

Aspergillus nidulans tef1 promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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TCARZ-SV40-GFP-PAmin-GF3-R4A22

Plasmid

#203769

Purpose

For production of template RNA for use in PRINT, GFP under SV40 promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for GFP

Use

Ivt template

Promoter

SV40

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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CTB

Plasmid

#141166

Purpose

Expresses recombinant Cholera Toxin B Subunit

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

CTB

Expression

Bacterial

Mutation

No secretion signal

Promoter

T7

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pDonor14-AcHELIX-KanR (CJT96)

Plasmid

#181794

Purpose

pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pVH607-Tier1-PCREm4-Nluc

Plasmid

#169594

Purpose

Tier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

cAMP response element-controlled NanoLuc Luciferase expression cassette

Expression

Mammalian

Promoter

PCREm4

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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xABEmax

Plasmid

#119813

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (xCas9)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(CCG3)

Plasmid

#235074

Purpose

Expresses Tad-CBE(CCG3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCG3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-c-Fos-(GGS)X6-HaloTag C58

Plasmid

#171437

Purpose

Expression of c-Fos-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

c-Fos-(GGS)X6-HaloTag C58

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAS1yl

Plasmid

#73226

Purpose

Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells

Depositor

Sheng Yang

Article

Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

Inserts

Cas9
sgRNA

Use

CRISPR; In y. lipolytica

Expression

Bacterial

Promoter

unknown

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMM134

Plasmid

#127215

Purpose

BeYDV replicon with constitutive Luc expression, IPT, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, IPT, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CYP2D6

Plasmid

#67090

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CYP2D6 (CYP2D6 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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NbV5-HALOTAG

Plasmid

#201482

Purpose

Indirect detection of V5-tagged protein using the HaloTag protein labeling technology.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-HALOTAG

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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GI-Gal4 DBD

Plasmid

#42500

Purpose

GIGANTEA fused with Gal4 DNA binding domain (DBD), part of a light-inducible transcription factor

Depositor

Ricardo Dolmetsch

Article

Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

Insert

GIGANTEA fused with Gal4 DBD

Tags

Gal4 DNA binding domain (DBD)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pYL-kasOp-FnCas12a3

Plasmid

#177875

Purpose

Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

Depositor

Yunzi Luo

Article

Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

Insert

Streptomyces codon optimized the FnCas12a mutant EP16

Use

CRISPR

Promoter

kasOp*

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(ACC2)

Plasmid

#235061

Purpose

Expresses Tad-CBE(ACC2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACC2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYSD_kappa_mRFP

Plasmid

#217830

Purpose

Yeast surface display Fab vector with kappa light chain and mRFP

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Expression

Yeast

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEC2861 (pSpy1C-PgyrA_ffluc)

Plasmid

#218511

Purpose

replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Mutation

deletion of Plac_mrfp cassette

Promoter

PgyrA

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pTPH418b

Plasmid

#185725

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

gIII(N)-NpuN-NpuC-gIII(C)

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-BFLF1

Plasmid

#162657

Purpose

Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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