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Tol2-lyzC-microRNA 7a-2-Dendra2

Plasmid

#97102

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 7a 2 Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 24-1-Dendra2

Plasmid

#97106

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 24 1 Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 31-Dendra2

Plasmid

#97107

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 31 Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 96--dendra2

Plasmid

#97108

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 96 dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 99-1-Dendra2

Plasmid

#97109

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 99 1 Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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FH95pUP95Q15AD45_64GW (D8)

Plasmid

#74031

Purpose

lentiviral expression of Psd95 shRNA and Psd95-Q15A _45_64-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Q15A, D45_64

Promoter

H1

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only
FH95pUP95L460pGW (E2)

Plasmid

#74032

Purpose

lentiviral expression of Psd95 shRNA and Psd95-L460P-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

L460P

Promoter

H1

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only
FH95pUP95DSKIrGW (D2)

Plasmid

#74025

Purpose

lentiviral expression of Psd95 shRNA and Psd95dSK and EGFP

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

delta SK

Promoter

H1

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only
pRS406 ATG9-GFP T119D

Plasmid

#114668

Purpose

Expresses yeast ATG9 (T119D) under the endogenous promoter with a GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Promoter

ATG9

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-RFP-miR-30 backbone

Plasmid

#97147

Purpose

As a control of miRNA expression

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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miR-722-Dendra pCDNA

Plasmid

#97163

Purpose

luciferase assay

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pTC102

Plasmid

#91176

Purpose

a derivative of pDIRECT_37J with translation initiation sequence optimized for dicots

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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FH95pUP95Q15AGW (D3)

Plasmid

#74026

Purpose

lentiviral expression of Psd95 shRNA and Psd95-E15R-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Q15A

Promoter

H1

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only
FH95pUP95E17RW (E8)

Plasmid

#74038

Purpose

lentiviral expression of Psd95 shRNA and Psd95 E17R (no GFP)

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

E17R

Promoter

H1

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
FH95pUP95Q15AW (E7)

Plasmid

#74037

Purpose

lentiviral expression of Psd95 shRNA and Psd95 Q15A (no GFP)

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Q15A

Promoter

H1

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
FH95pUP95GpW (E4)

Plasmid

#74034

Purpose

lentiviral expression of Psd95 shRNA and Psd95-GFPpren fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
FH95pUP95E17RGW (D4)

Plasmid

#74027

Purpose

lentiviral expression of Psd95 shRNA and Psd95-Q15A-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

E17R

Promoter

H1

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pTH1-SaSrtA-A

Plasmid

#64979

Purpose

Expresses the E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E108A

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-K

Plasmid

#64978

Purpose

Expresses the E105K mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pGGF004

Plasmid

#48845

Purpose

Provides a D-alanine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

p35S::D-AlanineR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site in 35S promoter re…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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