We narrowed to 11,556 results for: Vars;

1. pAAV-EF1a-ArgiNLS-EGFP

   Plasmid

   #220594

   Purpose

   Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   EGFP
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Luc-PS9

   Plasmid

   #177942

   Purpose

   Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   Luc2 (ORF1ab )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKrox24(MapErk)DsRed

   Plasmid

   #200114

   Purpose

   Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   MapErk sequence in minimal promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   5 copies of designed MapErk sequence, no other pr…

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)

   Plasmid

   #188385

   Purpose

   Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57-CMV-MAAP2

   Plasmid

   #218244

   Purpose

   1) Ectopic expression of MAAP2 protein in mammalian cells. 2) Packaging MAAP2 sequence into lentivirus for stable expression in mammalian cells.

   Depositor

   David Schaffer

   Article

   Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

   Insert

   Membrane-Associated Accessory Protein from Adeno-Associated Virus 2 driven by CMV promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

   Plasmid

   #167296

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   BAX (BAX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-ArgiNLS-mGreenLantern

   Plasmid

   #220596

   Purpose

   Constitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   mGreenLantern
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBpaRS3tRNA

   Plasmid

   #197102

   Purpose

   To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

   Depositor

   Kensaku Sakamoto

   Article

   Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

   Insert

   TyrRS variant, amber suppressor tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

   Promoter

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6)

   Plasmid

   #188383

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Pax6(DBD)-p65(428-551)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRR.1

    Plasmid

    #69995

    Purpose

    Mammalian expression of cytochrome p450 CYP2B6

    Depositor

    Kathrin Klein

    Article

    Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

    Insert

    CYP2B6 (CYP2B6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-EGFP-C-IFT88

    Plasmid

    #218733

    Purpose

    Expresses N-terminally EGFP-tagged IFT88 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

    Insert

    IFT88 (IFT88 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-RET-V5/HIS

    Plasmid

    #205606

    Purpose

    Expression of human RET receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    RET receptor tyrosine kinase (RET Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCherry N1 TOMM20

    Plasmid

    #157761

    Purpose

    Expression of TOMM20:mcherry in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCWXPGR-pTF-Wnt3a

    Plasmid

    #114287

    Purpose

    TET-inducible expression of Wnt3a

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    Wnt3a (Wnt3a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 P-cad

    Plasmid

    #47502

    Purpose

    Expresses P-cadherin in mammalian cells

    Depositor

    Alice Wong

    Article

    Cheung et al Oncogene. 2010 Apr 22;29(16):2427-40. do

    Insert

    cadherin 3, type 1, P-cadherin (placental) (CDH3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. GB1-A11(2-88)-Strep

    Plasmid

    #196214

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-88)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEMS2022

    Plasmid

    #49129

    Purpose

    AAV plasmid with DBH promoter (PRS2/3:Con) driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-PRS2/3:Con-iCre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    DBH

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAAV-MPRA-MluI-SpeI-EcoRI

    Plasmid

    #190196

    Purpose

    Empty vector for inserting MPRA libraries into AAV

    Depositor

    Hyejung Won

    Article

    McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

    Type

    Empty backbone

    Use

    AAV; Massively parallel reporter assay (mpra)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL5.0-MRLC-DD-GFP

    Plasmid

    #187260

    Purpose

    Lentiviral expression of myosin regulatory light chain-DD mutant

    Depositor

    Alpha Yap

    Article

    Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    T18D, S19D

    Promoter

    5' LTR

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLL5.0-MRLC-AA-GFP

    Plasmid

    #187259

    Purpose

    Lentiviral expression of myosin regulatory light chain-AA mutant

    Depositor

    Alpha Yap

    Article

    Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    T18A,S19A

    Promoter

    5' LTR

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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