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pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)

Plasmid

#134337

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA16_Efa

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3_V5-APEX2-Sec61B

Plasmid

#83412

Purpose

Expressed N-term tagged APEX2 on Sec61B in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

V5-APEX2-Sec61B (SEC61B Human, Synthetic)

Tags

APEX2 and V5

Expression

Mammalian

Promoter

CMV-F

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR-L26A,F27A

Plasmid

#89084

Purpose

mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

hAR FxxLF mutant (AR Human)

Expression

Mammalian

Mutation

hAR-L26A,F27A

Promoter

CMV

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
pEGFP-GNB1

Plasmid

#133856

Purpose

Mammalian expression of GNB1 N-terminally tagged with EGFP

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

GNB1 (GNB1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-DNAJC5

Plasmid

#205727

Purpose

Mammalian expression of DNAJC5

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Lentiviral

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR-F23A

Plasmid

#89089

Purpose

mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

hAR-FxxLF mutant (AR Human)

Expression

Mammalian

Mutation

hAR-F23A

Promoter

CMV

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
EYFP-hDF-deltaTM-pCMV-9

Plasmid

#72586

Purpose

human dysferlin no transmembrane domain tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin (DYSF Human)

Tags

EYFP

Expression

Mammalian

Mutation

transmembrane domain deleted

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-MAPTau-N-10

Plasmid

#55312

Purpose

Localization: Microtubules, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

MAPTau (MAPT Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
TetO-FUW-DN-REST

Plasmid

#61533

Purpose

Expresses DN-REST in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

REST (REST Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P73, Dominant negative REST (contains the Zf DNA-…

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pJR019

Plasmid

#198834

Purpose

Inactivation of G protein subunits through Pertussis toxin (PTX)

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::PTX-SL2-GFP::let-858 3'UTR

Expression

Worm

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-LC3

Plasmid

#38195

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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6599-SUMO1GG

Plasmid

#13377

Depositor

Anne Dejean

Article

Seeler et al Mol Cell Biol. 2001 May . 21(10):3314-24

Insert

SUMO1 (SUMO1 Human)

Expression

Mammalian

Mutation

mature form ending with GG

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-mAtg5

Plasmid

#38196

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pSC-PreE2

Plasmid

#52264

Purpose

Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linker

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

Ubc9 GST fusion (UBE2I Human)

Tags

GST

Expression

Bacterial

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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GAL-AR-1-503

Plasmid

#89093

Purpose

mammalian expression of human androgen receptor N-terminal 1-503 amino acids linked to GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Langley et al J Biol Chem. 1995 Dec 15;270(50):29983-9

Insert

hAR aa 1-503 (AR Human)

Tags

GAL4 DBD

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
pRR-AR-5Z

Plasmid

#23058

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

AR (AR Human)

Expression

Yeast

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only
ERG_pCSdest

Plasmid

#53864

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ERG (ERG Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508)

Plasmid

#134332

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA16_Lmo

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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p5070 pcDNA4c hBrd4 (1047-1362)

Plasmid

#14449

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1047-1362) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1047-1362 of Brd4 (the CTD)

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pEYFP-hDF C2B

Plasmid

#72583

Purpose

human dysferlin C2B domain tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin C2B domain (DYSF Human)

Tags

EYFP

Expression

Mammalian

Mutation

C2B domain

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only