We narrowed to 10,234 results for: UTY

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pEF1a_CD MUT TET1

Plasmid

#124083

Purpose

Expression of mutated catalytic domain only form of TET1

Depositor

Silvia Monticelli

Article

Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

Insert

TET1 - catalytic domain mutated (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Mutation

H1672Y, D1674A

Promoter

EF1 alpha

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEND-229_Cacnes_EforCP

Plasmid

#225615

Purpose

Replicative vector for red fluorescent reporter protein eForCP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+eForCP

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

EforCP

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for Cutibacterium acnes

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET-Duet1_6xHis-TEV-GABARAP-Gly

Plasmid

#190930

Purpose

Plasmid for the expression and purification of GABARAP. Internal Reference: SMC894

Depositor

Sascha Martens

Article

Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

Insert

GABARAP (GABARAP Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Ends with Glycine 116

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGP18 (CK2alpha' K69M)

Plasmid

#27090

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' K69M (CSNK2A2 Human)

Tags

HA

Expression

Mammalian

Mutation

K69M

Promoter

CMV

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pEND-226_Cacnes_mCitrine

Plasmid

#225613

Purpose

Replicative vector for green fluorescent reporter protein mCitrine strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCitrine

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

mCitrine

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for Cutibacterium acnes

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA

Plasmid

#178518

Purpose

For making Rabies virus SAD B19 strain L cell line with hyPBase

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

B19L-IRES-mCherry

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMG81-MBP-t-M.MpeI-N374K-His

Plasmid

#197985

Purpose

Expresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.

Depositor

Rahul Kohli

Article

Wang et al Nat Chem Biol. 2023 Jun 15. doi: 10.1038

Insert

MBP-t-M.MpeI-N374K-His

Tags

His and MBP

Expression

Bacterial

Mutation

M.MpeI-N374K

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pNCST-mAvicFP1

Plasmid

#129509

Purpose

Expresses mAvicFP1 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

mAvicFP1

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKO_LacZ_sgRNA

Plasmid

#74179

Purpose

lentiviral vector expressing sgRNA targeting LacZ

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

LacZ sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLXSN-Axl DN

Plasmid

#65223

Purpose

Expression of dominant negative Axl in mammalian cells

Depositor

Axel Ullrich

Article

Zhang et al Cancer Res. 2008 Mar 15;68(6):1905-15. d

Insert

AXL DN (AXL Human)

Use

Retroviral

Expression

Mammalian

Mutation

deleted cytoplasmic domain

Promoter

LTR

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
EGFP-Orf9b L52D

Plasmid

#165129

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Orf9b L52D

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAB07 (CK2beta)

Plasmid

#27091

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Tags

Myc

Expression

Mammalian

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only
pPB-CAG-3xFLAG::Flcn-pgk-hph

Plasmid

#48757

Purpose

PiggyBac backbone, CAG promoter driving murine 3xFLAG-Flcn fusion, Hygromycin selection

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

3xFLAG::Flcn (Flcn Mouse)

Use

Piggybac

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA5 TEX264wt-cSSH

Plasmid

#220209

Purpose

Mammalian expression of wild type TEX264, C-terminal twin-Strep_HA tag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Strep-strep-HA

Expression

Mammalian

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-ITR-EcYtR(NGS-6)-mCherry

Plasmid

#217363

Purpose

Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

E. coli tyrosine tRNA mutant, "NGS-6"

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK cyclin T1 (1-300) (P#432)

Plasmid

#14608

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-300) (CCNT1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-300

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pPPC011

Plasmid

#171143

Purpose

Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

Sp.pCas9, BBa_J23107

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)

Plasmid

#191650

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP1.v1 puro

Plasmid

#87790

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac (GST-preSci site-CD45)

Plasmid

#177889

Purpose

Baculo expression of GST tag fused to human CD45 ICD

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

CD45 (aa 598-1304 only) (PTPRC Human)

Tags

TEV-GST-PreScission cut site

Expression

Insect

Mutation

L652P

Promoter

Polyhedrin

Available Since

Aug. 24, 2024

Availability

Academic Institutions and Nonprofits only

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