We narrowed to 48,883 results for: des.1

1. M62 pPET28a-TEV-human beta cat domain N(1-137)

   Plasmid

   #17203

   Depositor

   Randall Moon

   Insert

   beta catenin (CTNNB1 Human)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   amino-terminal residues 1-137

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pGS275_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)]

   Plasmid

   #211891

   Purpose

   Bacterial expression of CENP-T[1-242/3D]

   Depositor

   Iain Cheeseman

   Insert

   CENP-T[1-242/3D] (CENPT Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T11D, T27D, T85D

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5

   Plasmid

   #61256

   Purpose

   CRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassette

   Depositor

   Frank Schnorrer

   Article

   Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

   Insert

   2xTY1-V5
   

   Use

   CRISPR

   Tags

   TY1 and V5

   Expression

   Mutation

   Promoter

   Available since

   Nov. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGS97_EGFP(A206K)-CENP-T(1-242)-I3-01 AAVS donor

   Plasmid

   #211866

   Purpose

   CENP-T(1-242)-I3-01 transgene integration at AAVS1 safe harbor locus

   Depositor

   Iain Cheeseman

   Insert

   CENP-T(1-242)-I3-01 (CENPT Human, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ8518 PB-PGK-N term (1-406) dLbCpf1-EE12RR345L-pA

   Plasmid

   #140221

   Purpose

   Constitutive PGK driven expression of N terminal dCpf1 (Cas12a) 406-split half with leucine zipper

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   dLbCpf1 (Cas12a) N terminal half (406) with zipper
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. EW416 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deImmLink-ZIK1KRAB

   Plasmid

   #236140

   Purpose

   Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sites

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   ZNF35(5-8)-ZNF250(1-4)-deImmunLink-ZIK1KRAB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter with two TetR binding sites

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. LSM041-3 SFFV-mCherry-SGc(stem-1(3xPP7))cSG-EGFP

   Plasmid

   #233408

   Purpose

   LIDAR reporter RNA with PCP binding site instead of MCP; mCherry as marker, EGFP as output

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   mCherry-SGc(stem-1(3xPP7))cSG-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. XZ392 SFFV-secNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP

   Plasmid

   #233415

   Purpose

   LIDAR reporter RNA; secreted NanoLuc Luciferase as marker, SEAP as output

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   secNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

   Plasmid

   #172065

   Purpose

   Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   mTagBFP2(11x7)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. -962 human cyclin D1 promoter AP-1 site mutant pGL3Basic

    Plasmid

    #32728

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase; Luciferase reporter gene

    Tags

    Expression

    Mutation

    -947 AP-1 binding site TGAGTCA deletion

    Promoter

    -962 CCND1 promoter

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

    Plasmid

    #159866

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. -962 human cyclin D1 promoter TCF (1) site mutant pGL3Basic

    Plasmid

    #32733

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -75 TCF(1) site CTTTGAT deletion

    Promoter

    -962 CCND1

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

13. LSM053 SFFV-mCherry-SGc(stem-1(3xPP7))cSG-mtagBFP (FLP-IN)

    Plasmid

    #233409

    Purpose

    LIDAR reporter RNA with PCP binding site instead of MCP; mCherry as marker, mTagBFP as output

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    mCherry-SGc(stem-1(3xPP7))cSG-mtagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

    Plasmid

    #159844

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid

    #159501

    Purpose

    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-2_PolyU(1)_HairpinDistance(10)nts
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6-27

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAV-U6+27-Linear-3_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid

    #159504

    Purpose

    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-3_PolyU(1)_HairpinDistance(10)nts
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6-27

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid

    #159499

    Purpose

    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-2_PolyU(1)_HairpinDistance(0)nts
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6-27

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAV-U6+27-Linear-1_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid

    #159495

    Purpose

    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-1_PolyU(1)_HairpinDistance(0)nts
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6-27

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

    Plasmid

    #101299

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-698 and 698-906 of alpha-C…

    Promoter

    Available since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

    Plasmid

    #86195

    Purpose

    U6 based expression of N meningitidis sgRNA

    Depositor

    Scot Wolfe

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    nmCas9 sgRNA cloning cassete
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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