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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,346 results for: multi

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Showing: 4881 - 4900 of 18346 results

  4. AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

    Plasmid
    #190112
    Purpose
    AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.
    Inserts
    MMLV-RT(dRH)
    U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
    Use
    AAV
    Tags
    bpNLS and bpNLS-P2A-eGFP
    Mutation
    mutations from RT in PE2 and truncation of RNAse …
    Promoter
    EFS and U6, H1
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    190112-plasmid-map-sequence-id-372717

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  5. PB-PE

    Plasmid
    #162796
    Purpose
    All-in-one prime editor piggyBac transposon
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    162796-plasmid-map-sequence-id-320034

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  7. Cas9-sgRNA-A+B

    Plasmid
    #149370
    Purpose
    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites
    Depositor
    Tarek Abbas
    Publication
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149370-plasmid-map-sequence-id-315474

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  8. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104910
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    104910-plasmid-map-sequence-id-206061

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  9. pRJPaph-bjGFP

    Plasmid
    #67032
    Purpose
    Plasmid for Paph-bjGFP integration downstream of B. diazoefficiens (B. japonicum) scoI
    Depositor
    Julia Vorholt
    Publication
    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    bjGFP
    Use
    Conjugative bacterial vector
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67032-plasmid-map-sequence-id-115543

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  15. cfSYFP2-N

    Plasmid
    #110631
    Purpose
    Cys-free yellow fluorescent protein for extracellular imaging. SYFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SYFP2.
    Depositor
    Ikuo Wada
    Insert
    cfSYFP2-N
    Tags
    Multi-cloning sites
    Expression
    Mammalian
    Mutation
    Cys48Ser and Cys70/Met
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    110631-plasmid-map-sequence-id-291941

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  16. pPV476

    Plasmid
    #42929
    Depositor
    James Lok
    Insert
    attR2::egfp (870 bp)::taa::Ss-era-1 terminator (598 bp)::attL3
    Use
    Multi-site gateway donor plasmid
    Available since
    March 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    42929-plasmid-map-sequence-id-58192

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  17. pPV475

    Plasmid
    #42928
    Depositor
    James Lok
    Insert
    attR2::Ss-era-1 terminator (598 bp)::attL3
    Use
    Multi-site gateway donor plasmid
    Available since
    March 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    42928-plasmid-map-sequence-id-58191

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  19. pAB1677 REPAIR.t3

    Plasmid
    #176320
    Purpose
    CMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)
    Depositor
    Feng Zhang
    Publication
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    human codon optimized Cas13bt3 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176320-plasmid-map-sequence-id-345287

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Showing: 4881 - 4900 of 18346 results