We narrowed to 19,256 results for: tec

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pTrex-Luc-Neo

Plasmid

#48336

Purpose

Trasgenically expresses firefly luciferase in T. cruzi with neomycin selection

Depositor

Rick Tarleton

Article

Sanchez-Valdez et al Elife. 2018 Mar 26;7. pii: 34039. doi: 1

Insert

firely luciferase

Use

Trypanasoma cruzi expression

Promoter

T. cruzi rRNA

Available Since

July 20, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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CyTERM-mGFP

Plasmid

#62237

Purpose

ER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.

Depositor

Erik Snapp

Article

Costantini et al Traffic. 2012 May;13(5):643-9. doi: 10.1

Insert

CyTERM-mGFP

Tags

mGFP

Expression

Mammalian

Mutation

signal anchor of rabbit cytochrome p450 and monom…

Promoter

CMV

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI

Plasmid

#115270

Purpose

Expresses TtCsm6 from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

TtCsm6

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTS2374-Tier1-PhCMV-Gal4_VP16

Plasmid

#169582

Purpose

Tier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

GAL4-transactivator

Expression

Mammalian

Promoter

PhCMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:(MCS)-S11-DI-GFP1–9

Plasmid

#108260

Purpose

Binary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19/mChe-Cs

Plasmid

#183171

Purpose

Construct for transient expression of cytoskeleton-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

mCherry-Cs

Tags

363 aa of A. thaliana FIMBRIN 1 (AtFIM1)

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pPB EF1a CCR6-eLOV-TEVcs-Gal4

Plasmid

#173119

Purpose

Expresses CCR6 eLOV receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-eLOV-TEVcs-Gal4

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre

Plasmid

#125574

Purpose

Expresses Cre under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Cre

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5 HA3-eDHFR-T2A-puro

Plasmid

#86395

Purpose

for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

Depositor

David Bentley

Article

Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

Insert

HA3 eDHFR T2A puro

Use

CRISPR

Tags

HA3 eDHFR T2A puro

Mutation

R12Y, G67S and Y100I mutations in E coli DHFR

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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MTK Lentivirus Multi Cassette Destination Vector (pAN2424)

Plasmid

#194221

Purpose

Backbone for constructing multi casette expression plasmids using the Mammalian Tookit

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET-APOBEC1-YTHmut

Plasmid

#200159

Purpose

For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

Depositor

Kate Meyer

Article

Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

Insert

APOBEC1-YTHmut

Tags

6xHis, HA, and MBP

Expression

Bacterial

Mutation

Codon optimized for E coli expression

Promoter

LacZ

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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tracrRNA(scr)-CJ8421_04975 mRNA

Plasmid

#199718

Purpose

Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 mRNA and scrambled tracrRNA

Use

CRISPR

Expression

Bacterial

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB508-cTP-sfGFP

Plasmid

#213863

Purpose

Binary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

Insert

Plastid transit peptide fused sfGFP

Expression

Bacterial and Plant

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPU-huTRIMmiR30-TRIM5

Plasmid

#132938

Purpose

All-in-one huTRIM5 rescue-TRIM5 shRNA knockdown

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

human TRIM5

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMM441

Plasmid

#61271

Purpose

Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.

Depositor

Timothy Lu

Article

Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

Insert

Cas9, tracrRNA, crRNA

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pJIT163-AFID-3

Plasmid

#167359

Purpose

For inducing predictable deletions in protoplasts and wheat plants

Depositor

Caixia Gao

Article

Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

Insert

A3A-Cas9-UDG-T2A-AP lyase

Expression

Plant

Mutation

WT

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BYG

Plasmid

#158126

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

mTagBFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pYC3.60-C1

Plasmid

#67899

Purpose

Yellow Cameleon 3.60 in a clontech-style backbone for expression in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

YC3.60

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only
pTH1-SaSrtA

Plasmid

#64973

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

T7 promoter

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pDonor14-ShoHELIX-KanR (BO4)

Plasmid

#181795

Purpose

pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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