We narrowed to 63,044 results for: cloning
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Plasmid#196293Purpose35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GPDepositorInsertFull length TSWV M antigenome encoding CBE in place of viral GP
UseCRISPRTags3×flagExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-NR5A1
Plasmid#192915PurposeBarcoded piggybac transposon vector with Dox-inducible expression of NR5A1DepositorAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Halo-Rab6A
Plasmid#190171PurposeExpresses HaloTag-Rab6A in mammalian cells.DepositorAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HaloTag-sec61b
Plasmid#186959PurposeExpress sec61b connected with N-terminus HaloTagDepositorAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_IRF1
Plasmid#167760PurposeExpresses IRF1 in mammalian cellsDepositorAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-noPS
Plasmid#177943PurposeTransient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.DepositorInserteGFP
ExpressionMammalianAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5-IMPROVED
Plasmid#154088PurposeImproved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells.DepositorUseLentiviralExpressionMammalianPromoterTet onAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2
Plasmid#166013Purposemammalian expression of MERS-CoV soluble spike trimer proteinDepositorInsertMERS-CoV S (spike)
Tags3C-His8-Strep2x2ExpressionMammalianPromoterCMV51Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS012: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)
Plasmid#163057PurposeGrx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)DepositorInsertGrx1-roGFP1-iEER
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30a-Spike-E
Plasmid#165096Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)
ExpressionBacterialAvailable sinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
MSCV-BCL6-t2A-BCL2
Plasmid#135305PurposeOverexpression of multiple human genes (BCL2 and BCL6)DepositorAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human J-chain
Plasmid#158215PurposeExpress the human J-chainDepositorAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RKS-Fzd7/8 subtype NGS Wnt
Plasmid#159628PurposeExpress Fzd7/8 subtype NGS Wnt in mammalian cellsDepositorInsertFzd7/8 subtype NGS Wnt
UseLentiviralTagsHis tagExpressionMammalianAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-His-SCov2-nsp7
Plasmid#154757PurposeExpression of SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
Tags6xHis-TEVExpressionBacterialPromoterT7Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
iOn-CAG∞RFP
Plasmid#154014PurposeVector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertmRFP1
ExpressionMammalianPromoterCAGAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-X1-Neo-DDRGK1-WT-HA
Plasmid#139845PurposeLentiviral expression of human DDRGK1-WT-HADepositorAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO-GAlpha15-RLuc8
Plasmid#140984PurposeEncodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlpha15-RLuc8 (GNA15 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha13-RLuc8
Plasmid#140986PurposeEncodes a G alpha subunit (GNA13) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlpha13-RLuc8 (GNA13 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-GfaABC1D-GRAB_Ado1.0
Plasmid#153288PurposeExpress the adenosine sensor GRAB_Ado1.0 in astrocytesDepositorInsertAdenosine sensor GRAB_Ado1.0
UseAAVPromoterGfaABC1DAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by