We narrowed to 59,537 results for: cloning

1. lentiGuide-mMaroon

   Plasmid

   #217008

   Purpose

   lentiviral plasmid for gRNA cloning with selectable mMaroon expression

   Depositor

   Deepta Bhattacharya

   Article

   D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMZ283

   Plasmid

   #52224

   Purpose

   Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments).

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Insert

   rrk1-sgRNA
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   rrk1

   Available Since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. p663-UBC-miniTurbo-V5-PIP5K1C_IDG-K

   Plasmid

   #170600

   Purpose

   Gateway destination clone of PIP5K1C (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-PIP5K1C (PIP5K1C Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p663-UBC-miniTurbo-V5-STK32C_IDG-K

   Plasmid

   #170610

   Purpose

   Gateway destination clone of STK32C (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-STK32C (STK32C Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRATIO1112

   Plasmid

   #141066

   Purpose

   Gateway cloning compatible dual fluorescent ratiometric binary vector

   Depositor

   David Nelson

   Article

   Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

   Type

   Empty backbone

   Tags

   mScarlet-I and mVenus

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOGG042

   Plasmid

   #113998

   Purpose

   Vector construction module. Tetracycline resistance module for construction of Golden Gate cloning vectors

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Tetracycline
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p663-UBC-miniTurbo-V5-NEK8_IDG-K

   Plasmid

   #189877

   Purpose

   Gateway destination clone of NEK8 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   NEK8 (NEK8 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCZGY2845

   Plasmid

   #87763

   Purpose

   Pnmr-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in subset of interneurons.

   Depositor

   Andrew Chisholm

   Article

   Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

   Insert

   Pnmr-1-PH-miniSOG
   

   Tags

   PH

   Expression

   Worm

   Promoter

   Pnmr-1

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. GGE085

   Plasmid

   #120738

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_Tef)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   P1_Tef
   

   Use

   Cloning/assembly plasmid

   Available Since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. hChT pcDNA3

    Plasmid

    #15766

    Depositor

    Randy Blakely

    Article

    Apparsundaram et al Biochem Biophys Res Commun. 2000 Oct 5.

    Insert

    hemicholinium-3-sensitive choline transporter (SLC5A7 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pICSL50008

    Plasmid

    #50314

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, GFP (A. victoria)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. GGE083

    Plasmid

    #120732

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Auxotrophy marker for selection after transformation in Y. lipolytica (Marker_URA3)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    Marker_URA3
    

    Use

    Cloning/assembly plasmid

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRATIO2212

    Plasmid

    #141302

    Purpose

    Gateway cloning compatible dual fluorescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Tags

    mScarlet-I and mVenus

    Expression

    Plant

    Promoter

    UBQ10 promoter

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. GGE002

    Plasmid

    #120739

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_GAPdh)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    P1_GAPdh
    

    Use

    Cloning/assembly plasmid

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. BRAF - A12G in pENTR

    Plasmid

    #216606

    Purpose

    gateway cloning donor vector containing BRAF ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    BRAF (BRAF Human)
    

    Use

    Gateway entry plasmid

    Mutation

    A12G

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBSSK mPTP-1B

    Plasmid

    #8603

    Depositor

    Ben Neel

    Article

    Chernoff et al Proc Natl Acad Sci U S A 1990 Apr;87(7):

    Insert

    PTP-1B (Ptpn1 Mouse)
    

    Expression

    Bacterial

    Available Since

    June 21, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pFH33

    Plasmid

    #125885

    Purpose

    Level0 Wheat TaU3 Pol III promoter with transcription Start-A

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    Level0 Wheat TaU3 Pol III promoter with transcription Start-A
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKER-mRuby2

    Plasmid

    #85977

    Purpose

    Expresses mRuby2 from the EF1a promoter in mammalian cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    mRuby2
    

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAK-EL-01

    Plasmid

    #125760

    Purpose

    Endlinker for tRNA-gRNA system when using only one guide

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    Endlinker
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAK-EL-04

    Plasmid

    #125763

    Purpose

    Endlinker for tRNA-gRNA system when using only four guides

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    Endlinker
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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