We narrowed to 38,228 results for: NAM

1. pLenti-PGK-flag-RAP1B-V12-puro

   Plasmid

   #156168

   Purpose

   Lentiviral vector for expression of flag-RAP1B-V12, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1B (RAP1B Bovine)
   

   Use

   Lentiviral

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   changed Glycine 12 to Valine

   Promoter

   human PGK

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-PGK-RAP1A-E63-neo

   Plasmid

   #156174

   Purpose

   Lentiviral vector for expression of RAP1A-E63, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1A (RAP1A Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   changed Glutamine 63 to Glutamic acid

   Promoter

   human PGK

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Epac-S-H94

   Plasmid

   #170341

   Purpose

   GFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

   Insert

   GFP(UST)_EPACdDEPCD_mCherry (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. miniTol2 x EF1a_EKAREN5(TA) + PGK-puro

   Plasmid

   #167819

   Purpose

   control sensor for EKAREN5

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5(TA)
   

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. B52 + CHEK2 sgSTOP

   Plasmid

   #100712

   Purpose

   B52 plasmid expressing CHEK2 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting CHEK2 (cloned using BbsI) (CHEK2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoters

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. B52 + FANCM sgSTOP

   Plasmid

   #100710

   Purpose

   B52 plasmid expressing FANCM sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting FANCM (cloned using BbsI) (FANCM Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoters

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. miniTol2 x EF1a_EKAREN4(TA) + PGK-blast

   Plasmid

   #167827

   Purpose

   Control sensor for EKAREN4

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4(TA)
   

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. miniTol2 x EF1a_EKAREN5(TA) + PGK-blast

   Plasmid

   #167820

   Purpose

   control sensor for EKAREN5

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5(TA)
   

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL4.23 C3_25

   Plasmid

   #60305

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   ROCK1 enhancer (ROCK1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pLenti-PGK-RAP2A-V12-neo

    Plasmid

    #156178

    Purpose

    Lentiviral vector for expression of RAP2A-V12, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-PGK-flag-RAP1B-V12-neo

    Plasmid

    #156175

    Purpose

    Lentiviral vector for expression of flag-RAP1B-V12, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-PGK-flag-RAP1B-N17-neo

    Plasmid

    #156176

    Purpose

    Lentiviral vector for expression of flag-RAP1B-N17, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 17 to Asparagine

    Promoter

    human PGK

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVX-CMV-XRCC1-gRNA res-Neo

    Plasmid

    #176149

    Purpose

    XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    X-ray repair cross complementing 1 (XRCC1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Dual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…

    Promoter

    CMV

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL3E-hGPR56 e1m promoter normal

    Plasmid

    #52298

    Purpose

    Reporter for normal human GPR56 exon 1m promoter activity. Human GPR56 e1m promoter (chr16:56,230,630-56,230,943 (hg18)) was inserted into pGL3E.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
    

    Use

    Luciferase

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. miniTol2 x EF1a_EKAREN4 + PGK-puro

    Plasmid

    #167824

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Epac-S-H150

    Plasmid

    #170345

    Purpose

    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

    Insert

    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-PGK-RAP2A-N17-neo

    Plasmid

    #156179

    Purpose

    Lentiviral vector for expression of RAP2A-N17, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    changed Serine 17 to Asparagine

    Promoter

    human PGK

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV x EF1a_EKAREN4(TA)-ires-puro

    Plasmid

    #167830

    Purpose

    Control sensor for EKAREN4

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4(TA)
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL4.23 C3_9

    Plasmid

    #60295

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    NKX6-1 enhancer (NKX6-1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. miniTol2 x EF1a_EKAREN4(TA) + PGK-puro

    Plasmid

    #167826

    Purpose

    Control sensor for EKAREN4

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4(TA)
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge