We narrowed to 29,658 results for: REP

1. pAG416GAL-mRuby3

   Plasmid

   #166141

   Purpose

   mRuby3 expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   mRuby3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG426GAL-ccdB-mRuby3

   Plasmid

   #166142

   Purpose

   Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a 2um element for high copy number when in yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST17-His10-ccdB-SNAP

   Plasmid

   #166146

   Purpose

   Gateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   His10 and SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST17-His10-Smt3-ccdB

   Plasmid

   #166147

   Purpose

   Gateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST17-His10-Smt3-ccdB-SNAP

   Plasmid

   #166148

   Purpose

   Gateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-Smt3 and SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST17-His10-Smt3-DDX5(kf)-SNAP

   Plasmid

   #166149

   Purpose

   Expression of N. furzeri DDX5 in E. coli

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   DDX5
   

   Use

   Tags

   His10-Smt3 and SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST17-His10-DDX5(kf)-SNAP

   Plasmid

   #166152

   Purpose

   Expression of N. furzeri DDX5 in E. coli

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   DDX5
   

   Use

   Tags

   His10 and SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLC045 - pL0_CrGLK (CDS1ns)

   Plasmid

   #203904

   Purpose

   MoClo compatible L0 plasmid containing the CrGLK coding sequence, lacking a stop codon to allow for C-terminal fusions.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   CrGLK (without a stop codon)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Silent mutation of BpiI or BsaI cut site for MoCl…

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLC047 - pL2_p2x35s-CrGLK-tMAS

   Plasmid

   #203906

   Purpose

   Overexpression of the CrGLK coding sequence from C. roseus in a plant binary vector for Agrobacterium-mediated transformation.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   CrGLK
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Silent mutation of BpiI or BsaI cut site for MoCl…

   Promoter

   CaMV 2x35S

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB278 - pL0_pT16H2 (pro + 5U)

    Plasmid

    #203889

    Purpose

    T16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    T16H2 promoter and 5'UTR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutation of BpiI or BsaI cut site for MoClo domes…

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUN1224 - pL0_pT3R (pro + 5U)

    Plasmid

    #203892

    Purpose

    T3R promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    T3R promoter and 5'UTR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutation of BpiI or BsaI cut site for MoClo domes…

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUN1215 - pL0_pDAT (pro + 5U)

    Plasmid

    #203895

    Purpose

    DAT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    DAT promoter and 5'UTR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutation of BpiI or BsaI cut site for MoClo domes…

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGMC00036 (aka pMC0223)

    Plasmid

    #212659

    Purpose

    Guide RNA targeting SERPINB3

    Depositor

    Raj Chari

    Article

    Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

    Insert

    SERBPINB3 guide RNA (SERPINB3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGMC00033 (aka pMC0224)

    Plasmid

    #210750

    Purpose

    Guide RNA targeting SERPINB3

    Depositor

    Raj Chari

    Article

    Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

    Insert

    SERBPINB3 guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

    Plasmid

    #189633

    Purpose

    Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro

    Plasmid

    #189636

    Purpose

    Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

    Plasmid

    #189632

    Purpose

    Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lnig-INTR-GFP

    Plasmid

    #208904

    Purpose

    encodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius niger, tagged with GFP

    Depositor

    Christian Gruber

    Article

    Di Giglio et al Sci Rep. 2017 Feb 1;7:41002. doi: 10.103

    Insert

    INTR
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRetroX-TRE3G_NT-GSDMD_I105N__C192A

    Plasmid

    #201176

    Purpose

    Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D N-terminal domain (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    aa1-276 only, I105N, C192A

    Promoter

    TRE3G

    Available since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRetroX-TRE3G_NT-GSDMD_C192A

    Plasmid

    #201172

    Purpose

    Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D N-terminal domain (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    aa1-276 only, C192A

    Promoter

    TRE3G

    Available since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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