167,318 results

1. F40F8.11 RNAi

   Plasmid

   #186731

   Purpose

   Knockdown of F40F8.11 gene

   Depositor

   Seung-Jae Lee

   Insert

   F40F8.11 targeting RNA
   

   Use

   RNAi

   Available Since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-Spe4-RfA-miRFP713-stop

   Plasmid

   #186391

   Purpose

   Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjection

   Depositor

   Patrick Lemaire

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-SKII-3XHA-eDHFR-NAT

   Plasmid

   #188926

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-eDHFR-NAT
   

   Expression

   Yeast

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB-Halo,IRES-NGFR

   Plasmid

   #164520

   Purpose

   All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_2pabPAC(Y72L)

   Plasmid

   #158970

   Purpose

   A negative control for AAV-mediated 2P-activatable bPAC

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

   Insert

   2pabPACY72L
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro

   Plasmid

   #120565

   Purpose

   Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K, T35A

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro

   Plasmid

   #120567

   Purpose

   Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K, Y40A

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX-uORF-HA-birA*-N-Ras(Q61K/E37A)-IRES-Puro

   Plasmid

   #120566

   Purpose

   Expresses HA-birA*-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K, E37A

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Xl-VSP1 R152Q

   Plasmid

   #51881

   Purpose

   expression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cells

   Depositor

   Laurinda Jaffe

   Article

   Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

   Insert

   VSP1 R152Q (tpte2 Frog)
   

   Use

   Xenopus oocyte expression

   Expression

   Mammalian

   Mutation

   R152Q: near the extracellular side of the fourth …

   Promoter

   CMV

   Available Since

   May 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFLAG_WT MDA5

    Plasmid

    #216798

    Purpose

    Expresses wild type (wt) melanoma differentiation-associated protein 5

    Depositor

    Sun Hur

    Insert

    melanoma differentiation-associated protein 5 (IFIH1 Human)
    

    Expression

    Mammalian

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV9)

    Viral Prep

    #20297-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    EF1a

    Tags

    mCherry (Cre-dependent)

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

12. CMV-VPR-S-L1-dCjCas9 MiniCAFE

    Plasmid

    #169910

    Purpose

    Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    MiniCAFE
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D8A, HNH-truncation (Δ495–609 aa) in CjCas9

    Promoter

    CMV

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AA364

    Plasmid

    #215956

    Purpose

    Fragmid fragment: (guide cassette) epegRNA expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    dU6:3_v1; BsmBI_v14; BsmBI_v15; evopreQ1_v1 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMVR8.74

    Plasmid

    #22036

    Purpose

    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Insert

    gag pol tat rev
    

    Expression

    Mammalian

    Available Since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-MBP-TEV

    Plasmid

    #69929

    Purpose

    bacterial expression of MBP-TEV n-terminal fusions

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    MBP and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV8)

    Viral Prep

    #105553-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    Syn

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pFU-GW-sMYH6-mNeon-TNNT2-mScarlet

    Plasmid

    #170712

    Purpose

    dual fluorescent reporter for MYH6 (short) and TNNT2 promoters

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Inserts

    mNeonGreen
    mScarlet
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TNNT2 and aMHC

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-HA-hIFITM1

    Plasmid

    #58399

    Purpose

    Expressed human IFITM1 with an N-terminal HA tag in mammalian cells

    Depositor

    Jacob Yount, Howard Hang

    Article

    Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

    Insert

    IFITM1 (IFITM1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-miRFP670nano3

    Plasmid

    #184664

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. MXS_BidirectionalCAG

    Plasmid

    #78175

    Purpose

    Bidirectional CAG promoter (compatible with the MXS Chaining Kit)

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    Bidirectional CAG Promoter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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