169,099 results

1. pv6_CBX1_2xChromo

   Plasmid

   #179393

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX1_2xChromo
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAH01Sp::Cas9

   Plasmid

   #128160

   Purpose

   Ptet-controlled expression of S. pyogenes Cas9

   Depositor

   Calvin Henard

   Article

   Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

   Insert

   Sp Cas9
   

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C1-FLAG-Ku70

   Plasmid

   #46957

   Purpose

   Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   Ku70 (XRCC6 Human)
   

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc

   Plasmid

   #234826

   Purpose

   STITCHR R2Tocc editor

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

   Insert

   nCas9h840a-xten-R2Tocc
   

   Use

   CRISPR

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME Lamin B Vhh mNeonGreen-pA (JDW 1312)

   Plasmid

   #224501

   Purpose

   Gateway compatible middle entry clone containing Lamin B nanobody fused to mNeonGreen with HA Tag (For visualizing the nuclear lamina, lamin chromobody)

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Insert

   Lamin B Vhh mNeonGreen-pA
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AiP11457 - pAAV-eHGT_078h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1457)

   Plasmid

   #163495

   Purpose

   Direct-expressing SYFP2 AAV Virus

   Depositor

   Allen Institute for Brain Science, Boaz Levi

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Promoter

   minBetaGlobin

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Ezrin-N-14

   Plasmid

   #55043

   Purpose

   Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Ezrin (EZR Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2582

   Plasmid

   #142436

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NR1D1 (NR1D1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. SUMO-PNGaseF

   Plasmid

   #228498

   Purpose

   10xHis-SUMO (Smt3) tagged PNGaseF (E. coli based protein expression)

   Depositor

   Richard Baker

   Insert

   ngl
   

   Tags

   10xHis-SUMO(Smt3)

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. SPLICS LY-MT Long P2A

    Plasmid

    #213613

    Purpose

    Detect the long-range Lysosome-Mitochondria contact

    Depositor

    Tito Calì

    Article

    Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

    Insert

    SPLICS LY-MT Long P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Str-Ii_VSVG-SBP-EGFP

    Plasmid

    #65300

    Purpose

    synchronize trafficking of VSVG from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    VSV-G
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGKB

    Plasmid

    #85077

    Purpose

    expression in Pichia pastoris

    Depositor

    Sheng Yang

    Article

    Yang et al Yeast. 2014 Apr;31(4):115-25.

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV x EF1a_EKAREN5(TA)-ires-puro

    Plasmid

    #167823

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. bact2-mKate2-P2A-TurboID-dVHH[GFP]

    Plasmid

    #163857

    Purpose

    Zebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBH

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    TurboID-VHH[EGFP]
    

    Use

    Zebrafish expression

    Tags

    mKate2-P2A-TurboID

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. YFP-DAT

    Plasmid

    #90228

    Purpose

    Expresses human DAT in mammalian cells

    Depositor

    Alexander Sorkin

    Article

    Sorkina et al J Biol Chem. 2003 Jul 25;278(30):28274-8

    Insert

    DAT (dopamine transporter) (SLC6A3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMyBADNT

    Plasmid

    #84690

    Purpose

    Protein expression in mycobacteria

    Depositor

    Matthias Wilmanns

    Article

    Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Promoter

    AraC

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett

    Plasmid

    #205703

    Purpose

    CMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Lyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N1 human cofilin WT

    Plasmid

    #50859

    Purpose

    expresses human cofilin fused to EGFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 (CFL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCVL SFFV-EF1s HA.NLS.Sce(opt)

    Plasmid

    #31479

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV/Ef1s HA NLS I-SceI
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Mutation

    "opt" indicates the coding sequence has…

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221_SLC19A1

    Plasmid

    #132266

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC19A1 (SLC19A1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 19, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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