We narrowed to 13,832 results for: Cas9

1. psBIG1a ActBT2ATetP2AP - mAGH

   Plasmid

   #229973

   Purpose

   Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-Hygro

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   ActBT2ATetP2AP - mAGH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. psBIG1a ActBT2ATetP2AP - H

   Plasmid

   #229972

   Purpose

   Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   ActBT2ATetP2AP - H
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. psBIG1a ActBT2AmcherryP2AP -TagBFPH

   Plasmid

   #229971

   Purpose

   Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   ActBT2AmcherryP2AP -TagBFPH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-dSa VP64 Afp

   Plasmid

   #99697

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV

   Depositor

   George Church

   Insert

   dCas9 and gRNA targeting Afp (Afp Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   VP64

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #222857

   Purpose

   This plasmid codes for the Slug-KH nickase with a sg(CTG)6

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   Slug-KHD10A+sgCTG
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, ITR2 and ITR2

   Expression

   Mammalian

   Mutation

   KH variant and D10A mutation

   Promoter

   H1 and eCMV

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #222859

   Purpose

   This plasmid codes for the Slug-KH nickase with a sg(AGC)6

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   Slug-KHD10A+sgAGC
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, ITR2 and ITR2

   Expression

   Mammalian

   Mutation

   KH variant and D10A mutation

   Promoter

   H1 and eCMV

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #222858

   Purpose

   This plasmid codes for the Slug-KH nickase with a sg(CAG)6

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   Slug-KHD10A+sgCAG
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, ITR2 and ITR2

   Expression

   Mammalian

   Mutation

   KH variant and D10A mutation

   Promoter

   H1 and eCMV

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC57-ITR2-H1-sgGCA-SlugD10A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #222860

   Purpose

   This plasmid codes for the Slug-KH nickase with a sg(GCA)6

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   Slug-KHD10A+sgGCA
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, ITR2 and ITR2

   Expression

   Mammalian

   Mutation

   KH variant and D10A mutation

   Promoter

   H1 and eCMV

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMLS713

   Plasmid

   #188885

   Purpose

   Multisite Gateway [4-3] Destination vector; miniMos transposon with lox2272-flanked Cbr-unc-119 marker

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Type

   Empty backbone

   Use

   Multisite gateway [4-3] destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOC5 cloning template vector

    Plasmid

    #183424

    Purpose

    FlpOFF knock-in vector

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOC3 cloning template vector

    Plasmid

    #183422

    Purpose

    CreON FlpOFF knock-in vector

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKEW395_AcrIIA14_truncated

    Plasmid

    #138284

    Purpose

    Expression of truncated AcrIIA14 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    truncated AcrIIA14
    

    Use

    CRISPR

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKEW353_AcrIIA13_truncated

    Plasmid

    #138282

    Purpose

    Expression of truncated AcrIIA13 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    truncated AcrIIA13
    

    Use

    CRISPR

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKEW398_AcrIIA15_truncated

    Plasmid

    #138286

    Purpose

    Expression of truncated AcrIIA15 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA15
    

    Use

    CRISPR

    Tags

    SFGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAP757-1

    Plasmid

    #99493

    Purpose

    6XHis::TEV::3XFlag::linker::Myc

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    6XHis::TEV::3XFlag::linker::Myc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAP605-1

    Plasmid

    #99482

    Purpose

    Myc::TEV::3XFlag::TEV::Myc

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    Myc::TEV::3XFlag::TEV::Myc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAP686-1

    Plasmid

    #99491

    Purpose

    TEV::eGFP::ER::V5::linker::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    TEV::eGFP::RE::V5::linker::3XFlag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAP640-2

    Plasmid

    #99487

    Purpose

    3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKER-Clover

    Plasmid

    #85974

    Purpose

    Expresses Clover from the EF1a promoter in mammalian cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    Clover
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef-1a

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2TAL3_DD_NvFP-7R_Ex2-LEFT

    Plasmid

    #90036

    Purpose

    TALEN-mediated mutagenesis of Exon 2 in NvFP-7R

    Depositor

    Matthew Gibson

    Article

    Ikmi et al Nat Commun. 2014 Nov 24;5:5486. doi: 10.

    Insert

    7R_Ex2-LEFT
    

    Use

    TALEN

    Tags

    3x Flag and Fok1-DD domain

    Expression

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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