We narrowed to 11,584 results for: VARS

1. Designed VEGF-binding scFv G6des13

   Plasmid

   #110213

   Purpose

   Yeast Surface Display of G6des13

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6des13
   

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95F

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

   Plasmid

   #139086

   Purpose

   Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAT105

   Plasmid

   #180510

   Purpose

   Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv2
   DpbCasX with R3 loop
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIYN3

   Plasmid

   #197099

   Purpose

   To express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.

   Depositor

   Kensaku Sakamoto

   Article

   Mukai et al Biochem Biophys Res Commun. 2011 Aug 12;

   Insert

   TyrRS variant, Nap3 tRNA
   

   Expression

   Bacterial

   Mutation

   Ala70, Thr158, Ser159, Arg286 (TyrRS)

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Designed VEGF-binding scFv G6des1

   Plasmid

   #110212

   Purpose

   Yeast Surface Display of G6des1

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6des1
   

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. NKP90 RFP (787)

   Plasmid

   #62001

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   NKP90 (TMEM126A Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   pgk

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pEMS1988

   Plasmid

   #49119

   Purpose

   AAV plasmid with CAGGS promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-CAGGS-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAV-hSyn-ASAP2s

   Plasmid

   #101276

   Purpose

   Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn (human synapsin)

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pαH-S-RRAR

   Plasmid

   #164569

   Purpose

   SARS-CoV-2 Spike protein with Furin site restored (S-RRAR variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-RRAR variant)
   

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_HCLS1-1/1

    Plasmid

    #91437

    Purpose

    Protein expression and purification of human SH3 domain construct HCLS1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    HCLS1-1/1 (HCLS1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3_GLP1R-V2R-NTEV-TCS-GV-2xHA

    Plasmid

    #194380

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Synthetic, Human)
    

    Tags

    2xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiU6-hASCL1 gRNA_1-MS2-Puro

    Plasmid

    #192670

    Purpose

    Lentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human ASCL1 activating gRNA #1 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEMS2021

    Plasmid

    #49128

    Purpose

    AAV plasmid with DCX (Ple302) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple302-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    DCX

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pJC5-4-insulator-BREreporter-insulator

    Plasmid

    #171499

    Purpose

    injections to make Bmp reporter mouse transgenic line

    Depositor

    James Briscoe

    Article

    Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. d

    Insert

    Insulator BRE-MLP:H2B-Turqoise
    

    Expression

    Mammalian

    Promoter

    BRE-MLP

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

15. pET-KIX (586-672)

    Plasmid

    #99336

    Purpose

    Bacterial expression of mouse CBP KIX domain

    Depositor

    Peter Wright

    Article

    Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

    Insert

    CBP KIX (586-672) (Crebbp Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-PSAT

    Plasmid

    #74115

    Purpose

    Expression of PSAT

    Depositor

    Lewis Cantley

    Article

    Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

    Insert

    pET28a-PSAT (PSAT1 Human)
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. KIF5C(1-560)-2xmCitrine-GFP11

    Plasmid

    #61667

    Purpose

    Tandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)
    

    Expression

    Mammalian

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. LV_EF1a_CDK4-P2A-Hygro_Barcode

    Plasmid

    #170223

    Purpose

    Barcoded lentiviral vector to express CDK4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Talin1 head-mCherry

    Plasmid

    #212005

    Purpose

    Expression of mouse talin1 head (1-490)-mCherry in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Cell Sci. 2019 Apr 3;132(7):jcs226514.

    Insert

    Mouse talin1 head domain (1-490) (Tln1 Mouse)
    

    Tags

    mCherry fluorescent protein

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-LYRa11-RBD-8h

    Plasmid

    #161822

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T324-D522)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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