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pFLxR1

Plasmid

#149496

Purpose

Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Chloramphenicol Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti puro HA-LMNB1

Plasmid

#227591

Purpose

Lentiviral plasmid expressing human LMNB1 protein

Depositor

Melina Fan, Addgene Research Program

Insert

LMNB1 (LMNB1 Human)

Use

Lentiviral

Tags

HA

Promoter

CMV

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCG144

Plasmid

#21376

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

mCherry in pDONR201

Use

Gateway Cloning

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pKNR5

Plasmid

#149466

Purpose

Broad-Host range expression vector, RK2 and pUC origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

NahR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PSalTTC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-natMX-tetO-Pcyc1-3xFLAG

Plasmid

#41021

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetO7-Pcyc1-3xFLAG

Use

N-terminal tagging/promoter replacement

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pMLS291

Plasmid

#73724

Purpose

SapTrap mCherry + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

mCherry + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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Zf Slit1b

Plasmid

#61280

Purpose

contains zebrafish Slit1b cDNA

Depositor

Chi-Bin Chien, Lara Hutson

Article

Hutson et al Dev Dyn. 2003 Nov;228(3):358-69.

Insert

Slit1b (slit1b Zebrafish)

Use

Cloning vector

Available Since

Dec. 16, 2014

Availability

Academic Institutions and Nonprofits only
PhdeA-cfp

Plasmid

#127940

Purpose

CFP fluorescent reporter for HdeA

Depositor

Mary Dunlop

Article

Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

Insert

PhdeA-cfp

Expression

Bacterial

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRU1105

Plasmid

#14469

Depositor

Philip Poole

Article

Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

Insert

DsRedT3

Expression

Bacterial

Available Since

April 23, 2007

Availability

Academic Institutions and Nonprofits only

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pMLS286

Plasmid

#73723

Purpose

Saptrap tagRFP + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

tagRFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pKCyR5

Plasmid

#149463

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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JDS70

Plasmid

#32285

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Type

Empty backbone

Use

T7

Tags

3X Flag and FOKI WT

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pILxR5

Plasmid

#149497

Purpose

Broad-Host range expression vector, pUC origin, Tn7 recognition sites, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMB2a

Plasmid

#190120

Purpose

Level 2 MoClo plasmid for A. baumannii

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pFLtR5

Plasmid

#167505

Purpose

Broad-Host range expression vector, RSF1010 origin, TetR repressor, PLtetO-1 promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TetR-RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLtetO-1

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP-ura4MX6

Plasmid

#33140

Purpose

genomic GFP tagging in yeast

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Inserts

ura4MX6
GFP

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pWD509

Plasmid

#73739

Purpose

Gateway compatible [1-2] Entry Vector encoding 2xNLS-FLP-D5

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

2xNLS-FLP-D5

Expression

Worm

Mutation

G5D

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pBLxR5

Plasmid

#167504

Purpose

Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDZ251 (pKAN 24xPP7SL cyc)

Plasmid

#35192

Depositor

Robert Singer

Article

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

24xPP7SL cyc

Available Since

Feb. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCD315

Plasmid

#113321

Purpose

pBAD-W108 scRNA.b1

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

scRNA.b1_MS2

Use

CRISPR

Expression

Bacterial

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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