We narrowed to 24,999 results for: Nes

1. pdCas9-M-4A6

   Plasmid

   #73434

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 4A6 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas9-M-C4

   Plasmid

   #73427

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor C4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSc1

   Plasmid

   #80436

   Purpose

   Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

   Depositor

   Ervin Welker

   Article

   Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

   Inserts

   sp Cas9 gRNA
   eGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available Since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. CHK2_HUMAN_D0

   Plasmid

   #79715

   Purpose

   This plasmid encodes the kinase domain of CHK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, John Chodera, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CHK2 (CHEK2 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pdCas9-M-3F2

   Plasmid

   #73428

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor C4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSc2

   Plasmid

   #80437

   Purpose

   Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

   Depositor

   Ervin Welker

   Article

   Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

   Inserts

   sp Cas9 gRNA
   eGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Bxb1Donor_G102H_ADRB2_LucReporter

   Plasmid

   #129794

   Purpose

   encodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

   Depositor

   Sriram Kosuri

   Article

   Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

   Insert

   ADRB2 (ADRB2 Human)
   

   Use

   Synthetic Biology

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   G102H

   Promoter

   N/A

   Available Since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe 3XFLAG-S37A-GATA6-3XAU1 puro

   Plasmid

   #72608

   Purpose

   For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutation

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA binding protein 6 (GATA6 Human)
   

   Use

   Retroviral

   Tags

   3XAU1 and 3XFLAG

   Expression

   Mammalian

   Mutation

   changed serine37 to alanine

   Promoter

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

9. MK08_HUMAN_D0

   Plasmid

   #79695

   Purpose

   This plasmid encodes the kinase domain of MK08. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, John Chodera, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MK08 (MAPK8 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-hSyn-iLMO (slGLuc-Mac-EYFP)

    Plasmid

    #114100

    Purpose

    fusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    Gaussia luciferase, superluminescent; Leptosphaeria maculans opsin; EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB-TR-TTHA

    Plasmid

    #232479

    Purpose

    Tetracycline inducible PiggyBac vector expressing bacterial TTHA1718 (TTHA) gene gene including flag-tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Thermus thermophilus HB8
    

    Use

    Transposon system

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-3xFLAG-TAF5-∆ex8

    Plasmid

    #209050

    Purpose

    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 deltaexon-8
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO inducible promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5-miniTurbo-TAF5-∆ex8

    Plasmid

    #209052

    Purpose

    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to TAF5 isoform lacking the alternative exon-8 (∆ex8)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 deltaexon-8
    

    Use

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO inducible promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5-3xFLAG-TAF5-FL_WobbleMut

    Plasmid

    #209055

    Purpose

    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full length TAF5 isoform mutated to be resistant to TAF5 siRNA

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 full length
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO inducible promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5-3xFLAG-TAF5-∆ex8_WobbleMut

    Plasmid

    #209056

    Purpose

    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 deltaexon-8
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO inducible promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-SET-EMD (N-SET-EMD)

    Plasmid

    #217765

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    SET (SET Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EMD domain (aa 70-226)

    Promoter

    CMV

    Available Since

    June 12, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pSET-EMD-EGFP (C-SET-EMD)

    Plasmid

    #217764

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    SET (SET Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EMD domain (aa 70-226)

    Promoter

    CMV

    Available Since

    June 12, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pEGFP-KSR1-CA3 (N-KSR)

    Plasmid

    #217755

    Purpose

    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400

    Promoter

    CMV

    Available Since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pKSR1-CA3-GS-mRFP1 (C-KSR-GS)

    Plasmid

    #217758

    Purpose

    Fluorescent reporter for ceramide (mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400 with GGSSGGGGA linker

    Promoter

    CMV

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKSR1-CA3-GS-EGFP (C-KSR-GS)

    Plasmid

    #217757

    Purpose

    Fluorescent reporter for ceramide (mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400 with GGSSGGGGA linker

    Promoter

    CMV

    Available Since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge