We narrowed to 32,724 results for: eng

1. FlagMBNL3

   Plasmid

   #96901

   Purpose

   expression of Flag-tagged human MBNL3

   Depositor

   Thomas Cooper

   Article

   Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

   Insert

   human MBNL3 cDNA (MBNL3 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ151-HDR

   Plasmid

   #113631

   Purpose

   Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

   Depositor

   Alex Kentsis

   Inserts

   Left homology arm
   Puromycin resistance gene
   Venus fluorescence gene
   Thymidine kinase
   Right homology arm
   

   Use

   Cre/Lox; Homology recombination selection vector

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a promoter, EF1a promoter (T2A cleaving sequen…

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQAT37

   Plasmid

   #223409

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQAT40

   Plasmid

   #223412

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV U6+27

   Plasmid

   #25709

   Purpose

   for nuclear expression of small RNAs in mammalian cells

   Depositor

   David Engelke

   Article

   Good et al Gene Ther. 1997 Jan . 4(1):45-54.

   Insert

   U6 snRNP (LSM2 Human)
   

   Expression

   Mammalian

   Available Since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-nArgBP2

   Plasmid

   #74514

   Purpose

   Express GFP-nArgBP2 fusion protein in mammalian cells

   Depositor

   Guoping Feng

   Article

   Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

   Insert

   nArgBP2 (Sorbs2 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSCARS1

   Plasmid

   #174868

   Purpose

   Contains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1

   Depositor

   Zengyi Shao

   Article

   Lopez et al Appl Microbiol Biotechnol. 2021 Aug 6. p

   Insert

   ARS1
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH1371

   Plasmid

   #199631

   Purpose

   Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFP

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   GPI
   

   Tags

   YFP

   Expression

   Worm

   Promoter

   Pregf-1

   Available Since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pTH1-SaSrtA

   Plasmid

   #64973

   Purpose

   Expresses the wild type of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Promoter

   T7 promoter

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFW2100

    Plasmid

    #224212

    Purpose

    Bacteroides genomic editing tool; IPTG inducible-Fncpf1

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Insert

    Fncpf1
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pre_II_CS3_7653_v2

    Plasmid

    #126855

    Purpose

    7653 b scaffold from compatibility group 3, made by method II, containing 1 Zn-dependent self-cleaving DNAzyme cassette for linearisation into 7560 b scaffold

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    II_CS3_7653_v2
    

    Use

    Synthetic Biology

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFPZFN1.4-B2H

    Plasmid

    #26791

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    GFP-ZFN1
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

13. GFPZFN2-B2H

    Plasmid

    #26792

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    GFP-ZFN2
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pZY102

    Plasmid

    #73933

    Purpose

    This plasmid was derived from pZY101 by inserting a GUS-intron reporter gene cassette into multiple cloning sites. This plasmid is recommended for use as empty control for service event request.

    Depositor

    Zhanyuan Zhang

    Article

    Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E

    Inserts

    bar
    GUS
    

    Expression

    Plant

    Mutation

    intron is inserted into the GUS coding region

    Promoter

    35S

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 HA-rnSOX9

    Plasmid

    #62972

    Purpose

    Expresses HA-tagged rat SOX9

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    SOX9 (Sox9 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. PclpB riboswitch GFP pZa

    Plasmid

    #170078

    Purpose

    GFP expression under the control of E. coli clpB promoter engineered with riboregulators

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PclpB

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFW5000

    Plasmid

    #224214

    Purpose

    E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQ

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pc3xTC20-alphaTubulin

    Plasmid

    #36327

    Depositor

    Amy Palmer

    Article

    Van Engelenburg et al Chembiochem. 2010 Mar 1;11(4):489-93.

    Insert

    alpha tubulin 1b with optimized tetracysteine peptides for ReAsH labeling (TUBA1B Human, Synthetic)
    

    Tags

    3xTC20

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-subtilase A

    Plasmid

    #35948

    Depositor

    Zhaoyang Feng

    Article

    Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

    Insert

    Subtilase A (subA E. Coli)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

20. 2a-copGFP(+HA)

    Plasmid

    #83806

    Purpose

    Express copGFP reporter for HDR-induced knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    copGFP
    

    Use

    Homologous recombination for gapdh loci

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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