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Showing: 961 - 1000 of 32928 results

  3. pComb3HSS

    Plasmid
    #64133
    Purpose
    2nd generation plasmid for phage display on modified geneIII, contains stuffer fragment
    Depositor
    Carlos Barbas
    Article
    Barbas et al Methods, A companion to Methods in Enzym
    Inserts
    SS Stuffer
    Light Chain Stuffer
    Heavy Chain Stuffer
    Use
    Phage display
    Tags
    geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64133-plasmid-map-sequence-id-119143

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  5. pAraH6HATT

    Plasmid
    #63901
    Purpose
    For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.
    Depositor
    Carlos Barbas
    Article
    Barbas et al Methods, A companion to Methods in Enzym
    Insert
    TT Fab
    Use
    Tags
    6xHis and HA tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    63901-plasmid-map-sequence-id-136457

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  6. pComb3HTT

    Plasmid
    #63900
    Purpose
    Backbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.
    Depositor
    Carlos Barbas
    Article
    Barbas et al Methods, A companion to Methods in Enzym
    Insert
    TT Fab
    Use
    Phage display
    Tags
    geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63900-plasmid-map-sequence-id-119142

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  15. pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA

    Plasmid
    #140014
    Purpose
    Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021
    Insert
    Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Y126A, Y197F
    Promoter
    EF1
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140014-plasmid-map-sequence-id-297743

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  17. pCMV-dCas13-inactive M3nls

    Plasmid
    #157854
    Purpose
    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
    Depositor
    David Liu
    Article
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Insert
    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
    Promoter
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    157854-plasmid-map-sequence-id-307627

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  19. pSBBi-RP CaDXMT

    Plasmid
    #139165
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Coffee arabica dimethylxanthine methyl transferase (CaDXMT) ; the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    3,7-dimethylxanthine N-methyltransferase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139165-plasmid-map-sequence-id-309355

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  20. pSBBi-RP CaMXMT

    Plasmid
    #139172
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica monomethylxanthine methyltransferase (CaMXMT); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    monomethylxanthine methyltransferase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139172-plasmid-map-sequence-id-309362

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  21. MTST5

    Plasmid
    #86602
    Purpose
    Custom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.
    Depositor
    Mary Ann Moran
    Article
    Satinsky et al Methods Enzymol. 2013;531:237-50. doi: 1
    Insert
    MTST5
    Use
    Internal standard reverse transcription
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Sept. 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    86602-plasmid-map-sequence-id-193516

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  22. MTST6

    Plasmid
    #86603
    Purpose
    Custom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.
    Depositor
    Mary Ann Moran
    Article
    Satinsky et al Methods Enzymol. 2013;531:237-50. doi: 1
    Insert
    MTST6
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    86603-plasmid-map-sequence-id-193515

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  33. pALS3-Ma-sfGFP-WT

    Plasmid
    #212119
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212119-plasmid-map-sequence-id-444185

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Showing: 961 - 1000 of 32928 results