Addgene

We narrowed to 1,608 results for: nif

Showing: 961 - 1000 of 1608 results

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L1P38

Plasmid

#195932

Purpose

Level 1 PhiC31 cassette with estradiol inducible promoter: p35S_XVE-PhiC31-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

p35S_XVE-PhiC31-tUBQ1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L1P47

Plasmid

#195934

Purpose

Level 1 PhiC31 cassette with constitutive promoter : pPP2AA3:PhiC31-NLS:t35S

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pPP2AA3:PhiC31-NLS:t35S

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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L1P1

Plasmid

#195917

Purpose

Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pPP2AA3-PhiC31-tUBQ1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pKEW395_AcrIIA14_truncated

Plasmid

#138284

Purpose

Expression of truncated AcrIIA14 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

truncated AcrIIA14

Use

CRISPR

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lenti

Plasmid

#138312

Purpose

Lenti expression of AcrIIA14, C-terminus

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA14, C terminus

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKEW353_AcrIIA13_truncated

Plasmid

#138282

Purpose

Expression of truncated AcrIIA13 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

truncated AcrIIA13

Use

CRISPR

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKEW398_AcrIIA15_truncated

Plasmid

#138286

Purpose

Expression of truncated AcrIIA15 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA15

Use

CRISPR

Tags

SFGFP

Expression

Bacterial

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHMGWA-Cas1

Plasmid

#122285

Purpose

Cas1 cloned in pHMGWA

Depositor

Jennifer Doudna

Article

Nunez et al Nat Struct Mol Biol. 2014 Jun;21(6):528-

Insert

Eco Cas1

Use

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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human EB1-GFP mutagenized Y44 Q46 both converted to A (JB140)

Plasmid

#39301

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Y44A and Q46A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pLBH531_MBP-Cas14a1 expression

Plasmid

#112500

Purpose

Expresses MBP fused to Cas14a1

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCX419

Plasmid

#229216

Purpose

All-in-one plasmid for labeling of XIST RNA via Csm complex

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Insert

Csm1; Csm2, Csm3(RNase mut)-sfGFP; Csm4; Csm5; Cas6; XIST crRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSHS212

Plasmid

#87346

Purpose

Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Use

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S

Promoter

T7

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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His_CL7_2NLS_iCas12a-4NLS

Plasmid

#222971

Purpose

Bacterial expression of NLS-rich iCas12a construct

Depositor

Jennifer Doudna

Article

Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

Insert

His_CL7_2NLS_iCas12a-4NLS

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETA-PAGFP

Plasmid

#11911

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Insert

pRSETA-PAGFP

Use

Tags

Expression

Bacterial

Mutation

The PAGFP has four mutations (L64F/T65S/V163A/T20…

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pBA787

Plasmid

#186242

Purpose

soc-C HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLBH533_MBP-Cas14b2 expression

Plasmid

#112506

Purpose

Expresses 10xHis-MBP fused to Cas14b2 under t7 promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b2

Use

CRISPR

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLBH532_MBP-Cas14b1 expression

Plasmid

#112505

Purpose

Expresses 10xHis-MBP fused to Cas14b1 under t7 promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b1

Use

CRISPR

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pKEW212-MBP-TEV-AcrVA4

Plasmid

#115658

Purpose

Expresses AcrVA4 in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Use

Tags

MBP-TEV

Expression

Bacterial

Mutation

Promoter

T7 RNAP

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCJH059_proD_Cas12c_DualRNA-GFP_CAM

Plasmid

#183076

Purpose

Plasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c tracrRNA and pre-crRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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JAB2121

Plasmid

#190343

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (empty vector control)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCJH019_SC101_pLAC_Cas12c(4)_Kan

Plasmid

#183071

Purpose

Wild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c_4

Use

Tags

Expression

Bacterial

Mutation

Wild-type

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pKEW189-MBP-TEV-AcrVA1

Plasmid

#115660

Purpose

Expresses AcrVA1 in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA1

Use

Tags

MBP-TEV

Expression

Bacterial

Mutation

Promoter

T7 RNAP

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCJH021_SC101_pLAC_Cas12c2_Kan

Plasmid

#183072

Purpose

Wild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA789

Plasmid

#186244

Purpose

soc-F HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA790

Plasmid

#186245

Purpose

dnap-C HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
L0-I18 Ub-Arg GG1-GG2

Plasmid

#195899

Purpose

Level 0 Ubiquitin-Arg tag, protein degration tag with GG1 GG2 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

Ub-Arg tag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW204

Plasmid

#113244

Purpose

E. coli expression vector for IHFa F13A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa F13A (ihfA E. coli)
IHFb

Use

Tags

Expression

Bacterial

Mutation

F13A

Promoter

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC_CRISPR_Cat

Plasmid

#120368

Purpose

Reporter plasmid for full-site selection assay

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

CRISPR_Cat

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA788

Plasmid

#186243

Purpose

soc-S HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF823_EFS-Cas9-PuroR_lenti

Plasmid

#138317

Purpose

Lenti expression of SpyCas9 with Puro selection

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SpyCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW206

Plasmid

#113246

Purpose

E. coli expression vector for IHFa K15A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa K15A (ihfA E. coli)
IHFb

Use

Tags

Expression

Bacterial

Mutation

K15A

Promoter

T7

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW212

Plasmid

#113234

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATC

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

GGTAG->CCATC

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
L1P63

Plasmid

#195938

Purpose

PhiC31-intein(DnaE-Npu) and Intein(DnaE-Ssp)-PhiC31 : pUBQ10-PI-tUBQ1-p35S-IP-t35S

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10-PI-tUBQ1-p35S-IP-t35S

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L0-I2 pPP2AA3-mutated GG0-GG1

Plasmid

#195891

Purpose

Level 0 pPP2AA3 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pPP2AA3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
L0-T1 Target PhiC31

Plasmid

#195890

Purpose

Level 0 PhiC31 target: attP_PhiC31-pUBQ10-attB_PhiC31 with golden gate linker in pBLUNT plasmid.

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 Target

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
L0-I38 BpiI-pUBQ10-BpiI

Plasmid

#195888

Purpose

Level 0 pUBQ10 promoter between BpiI sites for target construction

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
L1P5

Plasmid

#195920

Purpose

Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-NLS-DST-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pPP2AA3-PhiC31-NLS-DST-tUBQ1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
L1P19

Plasmid

#195924

Purpose

Level 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-NLS-DST-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10-PhiC31-NLS-DST-tUBQ1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
L1P24

Plasmid

#195927

Purpose

Level 1 PhiC31 cassette with constitutive promoter: p35S-PhiC31-NLS-DST-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

p35S-PhiC31-NLS-DST-tUBQ1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
L0-I9 PhiC31 GG1-GG4

Plasmid

#195892

Purpose

Level 0 PhiC31 integrase with GG1 and GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 integrase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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