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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 560 results for: DHFR

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Showing: 481 - 500 of 560 results

  1. pMT2-HA-RAPGEF2(S1244A/S1248A)

    Plasmid
    #110163
    Purpose
    Expression of human RAPGEF2 (S1244A/S1248A) in mammalian cells
    Depositor
    Daniele Guardavaccaro
    Publication
    Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    S1244A/S1248A
    Promoter
    CMV
    Available since
    June 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    110163-plasmid-map-sequence-id-211275

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  2. p1.1-Tr2-eGFP

    Plasmid
    #162782
    Purpose
    Fluorescent reporter for protein expression studies
    Depositor
    Ivan Vorobiev
    Publication
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enhanced green fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162782-plasmid-map-sequence-id-338334

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  3. pTM-RBDv2

    Plasmid
    #162785
    Purpose
    SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells
    Depositor
    Ivan Vorobiev
    Publication
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Insert
    SARS-CoV-2 S protein receptor binding domain (RBD) (S Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    Tags
    10xHis, c-myc, and hTPA leader
    Expression
    Mammalian
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162785-plasmid-map-sequence-id-333799

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  4. PB-tight-DDdCas9GFP-IRES-Neo

    Plasmid
    #102907
    Purpose
    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDdCas9GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102907-plasmid-map-sequence-id-198394

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  7. 3'-ecRESCUE-mCherry

    Plasmid
    #191486
    Purpose
    ecDHFR DD-mCherry, an inducible ecRESCUE RNA base editor: TMP-induced ecDHFR DD expression allows reversible control of the expression of dRanCas13b-ADAR2-mCherry
    Depositor
    Xu Ma
    Publication
    Wang et al Cell Commun Signal. 2021 Jul 31;19(1):81
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dRanCas13b-hADAR2-mCherry-ecDHFR (folA, ADARB1 Human, R. anatipestifer, E. coli)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    191486-plasmid-map-sequence-id-374351

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  12. pSc1-DD

    Plasmid
    #80439
    Purpose
    Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Publication
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Tags
    E. coli dihydrofolate reductase
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80439-plasmid-map-sequence-id-151455

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  18. SP080

    Plasmid
    #262958
    Purpose
    Barcoded Binding Assay (Barcode 0) with WT Omp25 TMD as the binding sequence
    Depositor
    Matthew Wohlever
    Insert
    3xHA-Sec61β soluble domain-Omp25TMD-3F4
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    262958-plasmid-map-sequence-id-523598

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  19. SP081

    Plasmid
    #262959
    Purpose
    Barcoded Binding Assay (Barcode 1) with WT Omp25 TMD as the binding sequence
    Depositor
    Matthew Wohlever
    Insert
    3xHA-Sec61β soluble domain-(SH3)1-Omp25TMD-3F4
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    262959-plasmid-map-sequence-id-523601

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  20. SP082

    Plasmid
    #262960
    Purpose
    Barcoded Binding Assay (Barcode 2) with WT Omp25 TMD as the binding sequence
    Depositor
    Matthew Wohlever
    Insert
    3xHA-Sec61β soluble domain-(SH3)2-Omp25TMD-3F4
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    262960-plasmid-map-sequence-id-523599

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Showing: 481 - 500 of 560 results