We narrowed to 14,582 results for: ESP
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Plasmid#191442PurposeExpresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locusDepositorInserthROSA26 sgRNA
UseCRISPRExpressionMammalianPromoterCBhAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuroN3xFLAG-His-CDK5RAP3
Plasmid#204555PurposeExpresses 3xFLAG-His-tagged CDK5RAP3 in mammalian cellsDepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
AW01_pU.CAG.eSpCas9(1.1).rBGpA
Plasmid#199255PurposeExpression construct encoding the high specificity eSpCas9(1.1) nucleaseDepositorInsertHigh specificity eSpCas9(1.1) nuclease
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationK848A K1003A R1060APromoterCAG promoterAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_NPM1_G6
Plasmid#178091PurposeExpresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.DepositorInsertNPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPRExpressionMammalianPromoterU6 promoterAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_ATP1A1_G3
Plasmid#86611PurposeExpresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmidDepositorInsertATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via hdr using ouabainExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-eYFP (AAV PHP.eB)
Viral Prep#117382-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagseYFPAvailable SinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Nesprin-3
Plasmid#175136PurposeLentiviral expression of V5-tagged mouse Syne3DepositorAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
KA2601_eSpCas9-G2P
Plasmid#124203PurposeExpression of eSpCas9(1.1) and sgRNADepositorTypeEmpty backboneExpressionMammalianAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEos2-CathespinB-6
Plasmid#57354PurposeLocalization: Lysosomes, Excitation: 505 / 569, Emission: 516 / 581DepositorAvailable SinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTFP1-CathespinB-6
Plasmid#55467PurposeLocalization: Lysosomes, Excitation: 462, Emission: 492DepositorInsertCathespinB
TagsmTFP1ExpressionMammalianPromoterCMVAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_NoFlag_hROSA26_Left
Plasmid#191442PurposeExpresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locusDepositorInserthROSA26 sgRNA
UseCRISPRExpressionMammalianPromoterCBhAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuroN3xFLAG-His-CDK5RAP3
Plasmid#204555PurposeExpresses 3xFLAG-His-tagged CDK5RAP3 in mammalian cellsDepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
AW01_pU.CAG.eSpCas9(1.1).rBGpA
Plasmid#199255PurposeExpression construct encoding the high specificity eSpCas9(1.1) nucleaseDepositorInsertHigh specificity eSpCas9(1.1) nuclease
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationK848A K1003A R1060APromoterCAG promoterAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-eSpCAS9-NG-pA
Plasmid#131466PurposeExpresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized eSpCas9-NG
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianAvailable SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dronpa-CathespinB-6
Plasmid#57265PurposeLocalization: Lysosomes, Excitation: 503, Emission: 518DepositorInsertCathespinB
TagsDronpaExpressionMammalianPromoterCMVAvailable SinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-T3-eSpCAS9-NG-pA
Plasmid#131466PurposeExpresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized eSpCas9-NG
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianAvailable SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dronpa-CathespinB-6
Plasmid#57265PurposeLocalization: Lysosomes, Excitation: 503, Emission: 518DepositorInsertCathespinB
TagsDronpaExpressionMammalianPromoterCMVAvailable SinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-deSpCas9-VP64-6xHis
Plasmid#92117PurposeExpression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive eSpCas9-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, H840A, K848A, K1003A, R1060APromoterT7Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
B-eSpCas9
Plasmid#126761PurposeExpression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.DepositorInsertB-eSpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationK848A; K1003A; R1060A; amino acids 1005-1013 repl…PromoterCBhAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Mesp2
Plasmid#72693PurposeDoxycyline regulated expression of Mesp2 in mammalian cellsDepositorAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only