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pCfB9360(IX-1_Markerfree_BackBone)

Plasmid

#161601

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site IX-1, (Chr IX: 388864..388883)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9362(XV-1_Markerfree_BackBone)

Plasmid

#161603

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XV-1, (Chr XV: 93186..93205)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAF7E1

Plasmid

#185839

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>Zif268>MED15>PABF1

Expression

Yeast

Mutation

None

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAF4EH4R

Plasmid

#185837

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>Zif268>VP16-PABF1

Expression

Yeast

Mutation

None

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

Plasmid

#165029

Purpose

Express cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C)

Plasmid

#165026

Purpose

Express cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaM8(N)-cpFRB1-T2A-FKBP-LaM8(C)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C)

Plasmid

#165028

Purpose

Express cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaM8(N)-cpFRB2-T2A-FKBP-LaM8(C)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMV47

Plasmid

#128866

Purpose

Position 1 module- IX301997 (yeast integrative region IX301997)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Yeast integrative region chrIX301997

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMV46

Plasmid

#128865

Purpose

Position 1 module- VI260998 (yeast integrative region VI260998)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Yeast integrative region chrVI260998

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0114

Plasmid

#117553

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_NbPDS1 N. benthamiana

Expression

Plant

Promoter

At U6-26 (pICSL90002)

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0029

Plasmid

#117541

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

crRNA (LbCas12a)

Expression

Plant

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0028

Plasmid

#117540

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

crRNA (AsCas12a)

Expression

Plant

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0014

Plasmid

#117524

Purpose

Level0 Golden Gate part: CDS, SpCas9-KA

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-KA (with stop codon)

Use

CRISPR

Mutation

K855A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0009

Plasmid

#117522

Purpose

Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (no stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL90005

Plasmid

#117520

Purpose

Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-h (no stop codon)

Use

CRISPR

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0008

Plasmid

#117535

Purpose

Level0 Golden Gate part: CDS, AsCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

AsCas12a (with stop codon)

Use

CRISPR

Tags

3xHA and NLS

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2336

Plasmid

#67541

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP4G

Plasmid

#104502

Purpose

Used to evaluate the expression output of Saccharomyces cerevisiae PGM2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

ScPGM2 promoter-yEGFP

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pILGFP4K

Plasmid

#104506

Purpose

Used to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SpGAL1 promoter-yEGFP

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pILGFP4O

Plasmid

#104510

Purpose

Used to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SpdGAL2 promoter-yEGFP

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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