Skip to main content
Addgene
Showing: 481 - 500 of 2456 results

  7. D. vulgaris sp2 CRISPR/pACYCDuet-1

    Plasmid
    #81186
    Purpose
    Expresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coli
    Depositor
    Jennifer Doudna
    Article
    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:
    Insert
    Synthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
    Use
    CRISPR
    Tags
    None
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    81186-plasmid-map-sequence-id-162820

    Enlarge

  13. SIAP-1-COMP-blac-flag-his

    Plasmid
    #111002
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    sporozoite invasion associated protein 1 (SIAP-1) (PF3D7_0408600 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111002-plasmid-map-sequence-id-224547

    Enlarge

  14. K-to-R mutant NPRL2(1-380) in pLJM60

    Plasmid
    #184562
    Purpose
    CMV-driven expression of an untagged mutant NPRL2 (core subunit of GATOR1) — native sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.
    Depositor
    Kacper Rogala
    Article
    Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:
    Insert
    NPRL2 (NPRL2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    All lysines were mutated to arginines.
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    184562-plasmid-map-sequence-id-376960

    Enlarge

Showing: 481 - 500 of 2456 results