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MSCV-PIG-N3FLAG-TAF10

Plasmid

#105990

Purpose

retrovirally express mouse TAF10 with 3*FLAG tag at N terminal, GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

FLAG tagged TAF10 (Taf10 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_SIK3_CR

Plasmid

#108104

Purpose

Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

change guanine 501 to cytosine (silent mutation)

Promoter

EFS promoter

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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gRNA_DNMT3b

Plasmid

#41823

Purpose

Expresses a guide RNA (gRNA) to target DNMT3b for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_DNMT3b (DNMT3B Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only
plKO.1-DNAJC5-ShRNA

Plasmid

#205729

Purpose

Knockdown of DNAJC5

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

shRNA targeting DNAJC5 (DNAJC5 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5 (WT)-HaloTag

Plasmid

#205710

Purpose

Mammalian expression of DNAJC5 (WT)-HaloTag

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pECE-M2-PAK2 S20A

Plasmid

#31681

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Use

Tags

M2

Expression

Mammalian

Mutation

changed Serine 20 to Alanine

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA4-RI1-332-MYC-His6

Plasmid

#185351

Purpose

Mammalian expression of Ribophorin I

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Ribophorin I (RPN1 Human)

Use

Tags

Myc, 6xHis

Expression

Mammalian

Mutation

aa 1-332 only

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST_5xUAS:EB3-mScarlet-I

Plasmid

#192358

Purpose

EB3 tagged with mScarlet-I, expressed under the UAS promoter

Depositor

Francesca Peri

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

EB3 (MAPRE3 Human)

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGL421_MLL_promoter

Plasmid

#118695

Purpose

Express MLL/KMT2A promoter for luciferase assay

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

MLL_promoter

Use

Luciferase

Tags

Expression

Mutation

Promoter

MLL/KMT2A promoter

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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782 pMEActMEK

Plasmid

#31166

Depositor

Nathan Lawson

Article

Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

Insert

MEK (MAP2K1 Human)

Use

Entry vector

Tags

Expression

Mutation

Ser218Glu Ser222Asp; activated MEKK

Promoter

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
FUW-M2-PAK2 S20A

Plasmid

#31664

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Use

Lentiviral

Tags

M2

Expression

Mammalian

Mutation

Changed Serine 20 to Alanine

Promoter

Available since

April 12, 2012

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ARAF_S214C

Plasmid

#51077

Purpose

mammalian expression of ARAF S214C

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

S214C

Promoter

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 delta 610-615

Plasmid

#19941

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 delta 610-615 (CUL1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

CUL1 with a ROC1 binding mutant

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pHAGE_puro_ZFP64_N_3XFLAG

Plasmid

#118693

Purpose

Express 3xflag tagged ZFP64 for flag ChIP experiments

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

ZFP64 (ZFP64 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_WT_ZFP64

Plasmid

#118661

Purpose

Express human wild type ZFP64 gene

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

ZFP64 (ZFP64 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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myc-Miro1 (lacking TM)

Plasmid

#127613

Purpose

Human Miro1 with the transmembrane deleted

Depositor

Josef Kittler

Article

Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

Insert

Miro1 (RHOT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Deletion of transmembrane domain

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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360 pCR kdrb

Plasmid

#13067

Depositor

Nathan Lawson

Article

Covassin et al Proc Natl Acad Sci U S A. 2006 Apr 25. 1

Insert

kdrb (kdr Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
LRG-2.1T-neo-sgROSA

Plasmid

#105587

Purpose

lentivirally express gRNA targeting ROSA26 locus with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting rosa26 locus

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 L756A/I757A

Plasmid

#19938

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 L756A/I757A (CUL1 Human)

Use

Tags

HA2

Expression

Mammalian

Mutation

Mutation of two adjacent hydrophobic residues, L…

Promoter

Available since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-PAK2 S20A

Plasmid

#31672

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

changed Serine 20 to Alanine

Promoter

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only