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pET14b – lambda exonuclease

Plasmid

#104531

Purpose

bacterial expression of lambda exonuclease

Depositor

Charles Bell

Article

Zhang et al Proc Natl Acad Sci U S A. 2011 Jul 19;10

Insert

λ exonuclease

Tags

His6 followed by a thrombin site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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mSA-phi29

Plasmid

#210815

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

mSA-Phi29

Expression

Bacterial

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-Vinculin tail

Plasmid

#107154

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

Insert

Vinculin (VCL Human)

Tags

GST

Expression

Bacterial

Promoter

Ptac

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLG023 (dsr2)

Plasmid

#157901

Purpose

Expresses the dsr2 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

dsr2 (SIR2)

Expression

Bacterial

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLG028 (hhe)

Plasmid

#157906

Purpose

Expresses the hhe defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

hhe (DUF4011-helicase-Vsr-DUF3320)

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSP6-EBNA(2A+DBD)

Plasmid

#98749

Purpose

In vitro transcription of modified EBNA1 mRNA

Depositor

Sara Howden, Jim Vadolas

Article

Howden et al Hum Gene Ther. 2006 Aug;17(8):833-44.

Insert

Modified EBNA1

Use

In vitro transcription

Promoter

SP6

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLG032 (ppl)

Plasmid

#157910

Purpose

Expresses the ppl defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

ppl (PHP-ATPase)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaD (pESC106)

Plasmid

#50388

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

PPAT

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-GFP

Plasmid

#175757

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GFP (Control for APEX experiments)

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

GFP

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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UbcH5B

Plasmid

#60443

Purpose

Express E2 UbcH5B in E. Coli

Depositor

Cynthia Wolberger

Article

Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

Insert

UbcH5B (UBE2D2 Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pT3TS-BASU-EGFP

Plasmid

#163845

Purpose

In vitro transcription of BASU-EGFP. Parton lab clone JRR

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

BASU

Use

In vitro transcription of rna

Tags

EGFP

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-HisYFP-Sp100

Plasmid

#53141

Purpose

E.coli expression of HisYFP tagged human Sp100 fragment

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

Sp100A fragment (SP100 Human)

Tags

His-YFP

Expression

Bacterial

Mutation

fragment (amino acids 71-480); deletion of L75

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) FLIPQTV3.0 2m

Plasmid

#63731

Purpose

FLIPQTV3.0 glutamine sensor with R75MW220A mutations

Depositor

Sakiko Okumoto

Article

Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

Insert

FLIPQTV3.0 sensor, 2mM affinity

Expression

Mammalian

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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MET-HYGRO

Plasmid

#218113

Purpose

Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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LYS-Leu2

Plasmid

#218114

Purpose

Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Flag TSC2 wt

Plasmid

#12132

Depositor

Cheryl Walker

Article

Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

Insert

TSC2 (TSC2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2006

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform II-EGFP

Plasmid

#120316

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the C-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLG022 (dsr1)

Plasmid

#157900

Purpose

Expresses the dsr1 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

dsr1 (SIR2-DUF4020)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-Septin 11 isoform II

Plasmid

#120318

Purpose

Septin 11 isoform II expression (mCherry-tag at the N-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-BsaI_pJMP2776

Plasmid

#208890

Purpose

Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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