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pDonor_Tn-pOUT

Plasmid

#213905

Purpose

Mariner transposon with outward-facing pJ23104 promoter

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and outward-facine pJ23104 promoter

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-lyzC

Plasmid

#107591

Purpose

An entry vector with a lyzC promoter

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

Lysozyme C promoter

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-Vector-Dendra2

Plasmid

#97101

Purpose

empty vector for overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Type

Empty backbone

Use

Zebrafish

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBE50

Plasmid

#174657

Purpose

CAPTURE method receiver plasmid for Bacillus cloning and heterologous expression

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Type

Empty backbone

Use

Synthetic Biology

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pB1H1

Plasmid

#12611

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

Insert

Dorsal RHR in pB1H1 (dl Fly)

Tags

flag

Expression

Bacterial

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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PEG-Actinin-M-sstFRET

Plasmid

#61100

Purpose

Expression of α-actinin with internal sstFRET inserted to measure cytoskeletal stress in vivo

Depositor

Fred Sachs

Article

Meng et al J Cell Sci. 2011 Jan 15;124(Pt 2):261-9.

Insert

α-actinin-sstFRET

Expression

Mammalian

Mutation

sstFRET inserted at position 300

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-non-targeting (pJK5)

Plasmid

#229052

Purpose

Cloning backbone for PspCas13b crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JP1520 PIK3CA WT HA

Plasmid

#14570

Depositor

Joan Brugge

Article

Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

Insert

PIK3CA (PIK3CA )

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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pmU1-CasRx-non-targeting (pKW83)

Plasmid

#229051

Purpose

Cloning backbone for CasRx crRNA (Mouse U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAC1821_pCR8-SMN2-DN-RG6-SA

Plasmid

#118655

Purpose

Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

SMN2-DN-RG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGGF005

Plasmid

#48846

Purpose

Provides a hygromycin resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pUBQ10::HygromycinR::tOCS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[sfGFP]

Plasmid

#213902

Purpose

Mariner transposon expressing sfGFP with kan selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and superfolder GFP

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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Filamin-C-stFRET

Plasmid

#61107

Purpose

Expression of Filamin with C-term stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pSIBR4-Cre

Plasmid

#219435

Purpose

Theophylline_induced self_splicing intron_based riboswitch for the moderate expression of Cre recombinase

Depositor

Sheng Yang

Article

Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

Insert

intron 4-Cre

Expression

Bacterial

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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Filamin-M-stFRET

Plasmid

#61105

Purpose

Expression of Filamin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pGGY001

Plasmid

#48866

Purpose

Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible plant transformation vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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CelTag Plasmid

Plasmid

#66562

Purpose

Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

Depositor

David Engelke

Article

Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

Insert

CelTag

Expression

Bacterial

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGGF003

Plasmid

#48844

Purpose

Provides a D-alanine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::D-AlanineR::tMAS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Actinin-M-stFRET

Plasmid

#61103

Purpose

Expression of α-actinin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2015

Availability

Academic Institutions and Nonprofits only

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pGGY003

Plasmid

#48867

Purpose

Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible plant transformation vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSuper-gmiR-1

Plasmid

#171553

Purpose

a human miR-1-expressing plasmid

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

miR-1-2 (MIR1-2 Human)

Expression

Mammalian

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGGA004

Plasmid

#48815

Purpose

Provides the 35S promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

35S promoter

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site removed by substit…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-luc-miR-122

Plasmid

#171557

Purpose

a positive control reporter with 3' UTR sequences perfectly complementary to miR-122

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

a sequences perfectly complementary to miR-122

Expression

Mammalian

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescript-Evf1

Plasmid

#99479

Purpose

Subcloning of the Evf1 long non-coding RNA

Depositor

Jhumku Kohtz

Article

Feng et al Genes Dev. 2006 Jun 1;20(11):1470-84. Ep

Insert

Evf1

Expression

Bacterial

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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3694 pMIG HA-Bax

Plasmid

#8806

Depositor

Stanley Korsmeyer

Article

Cheng et al Science 2003 Jul 25;301(5632):513-7.

Insert

Bax (Bax Mouse)

Use

Retroviral

Tags

GFP and HA

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
Actinin-C-stFRET

Plasmid

#61104

Purpose

Expression of α-actinin with C-term stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pGGF001

Plasmid

#48842

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::BastaR::tMAS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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B7N6P4

Plasmid

#163180

Purpose

Inducible expression of UniProt identifier B7N6P4

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

B7N6P4

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pXf20pemIK

Plasmid

#126577

Purpose

Vector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stability

Depositor

Lindsey Burbank, Drake Stenger

Article

Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

Type

Empty backbone

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-1 Sesn3

Plasmid

#61875

Purpose

bacterial expression of a GST-Sesn3 (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn3 (Sesn3 Mouse)

Tags

GST

Expression

Bacterial

Mutation

none

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only