We narrowed to 35,551 results for: Tat

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pWZL Blast H-Ras G12V T35S

Plasmid

#12278

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V T35S (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The T35S mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pWZL Blast H-Ras G12V Y40C

Plasmid

#12279

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V Y40C (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The Y40C mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pGWF1

Plasmid

#18856

Depositor

Wolf Frommer

Article

Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

Type

Empty backbone

Tags

CFP and Venus

Expression

Bacterial

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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pUDE735

Plasmid

#103024

Purpose

expression of a Cpf1 programming crRNA targetting CAN1, HIS4, PDR12 and ADE2 (crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAW212

Plasmid

#172196

Purpose

P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

Depositor

Chang Liu

Article

Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

Insert

p10B2-AT110

Expression

Yeast

Promoter

10B2 (P1 specific promoter)

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAX_WT_V5

Plasmid

#82944

Purpose

Gateway Donor vector containing MAX, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAX (MAX Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAW212

Plasmid

#172196

Purpose

P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

Depositor

Chang Liu

Article

Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

Insert

p10B2-AT110

Expression

Yeast

Promoter

10B2 (P1 specific promoter)

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ICUE4

Plasmid

#181846

Purpose

Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

ICUE4 (RAPGEF3 Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAX_WT_V5

Plasmid

#82944

Purpose

Gateway Donor vector containing MAX, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAX (MAX Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ICUE4

Plasmid

#181846

Purpose

Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

ICUE4 (RAPGEF3 Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE22

Plasmid

#90364

Purpose

STAT6 - Stat6 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

STAT6

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLT1

Plasmid

#116744

Purpose

Lentiviral expression of FLT1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FLT1 (FLT1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-PRSX8 eArchT3.0-EYFP

Plasmid

#89538

Purpose

Expresses eArchT 3.0-eYFP in Phox2 (including catecholamine) neurons

Depositor

Ruth Stornetta

Article

Burke et al J Physiol. 2015 Jul 1;593(13):2909-26. d

Insert

eArchT 3.0

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Promoter

PRSx8

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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L304-EGFP-Tubulin-K40R

Plasmid

#64059

Purpose

EGFP fused at the N-terminus of tubulin with a mutation K40R; lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pRS303-LacZ

Plasmid

#1169

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

LACZ

Tags

GAL1:10 promoter

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pLminP_Luc2P_RE22

Plasmid

#90364

Purpose

STAT6 - Stat6 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

STAT6

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLT1

Plasmid

#116744

Purpose

Lentiviral expression of FLT1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FLT1 (FLT1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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PLKO.1-G3BP1-CDS

Plasmid

#136039

Purpose

G3BP1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGGGAATTTGTGAGACAGTAT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRSetB-Peredox

Plasmid

#73806

Purpose

Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in bacteria, with N-terminal his-tag

Depositor

Gary Yellen

Article

Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

Insert

Peredox

Tags

his-tag to facilitate purification

Expression

Bacterial

Promoter

T7

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pJW1507

Plasmid

#62361

Purpose

pFA6a -mVenus-BirA::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

c-terminal mVenus-BirA tagging

Use

Yeast c-terminal tagging vector

Tags

mVenus-BirA

Mutation

L232H mutation compared to standard mVenus

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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