We narrowed to 8,178 results for: gnal

1. PMmEos3.2

   Plasmid

   #203755

   Purpose

   Encodes the membrane anchor of Lyn including the first 10 residues of the N terminus, with 1 myristoylation and 1 palmitoylation, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   Lyn n-term (LYN Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-JAK1Gly590Arg

   Plasmid

   #174573

   Purpose

   Human FLAG-tagged JAK1 Gly590Arg mutant expression (N-term. tag) (CMV prom.)

   Depositor

   Mathew Garnett

   Article

   Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

   Insert

   JAK1Gly590Arg
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Gly590Arg

   Promoter

   CMV

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRibo-Sec-APEX2m

   Plasmid

   #176844

   Purpose

   Expression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-Sec-APEX2m
   

   Use

   Inducible

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRAF_S259A_pcDNA3.1

   Plasmid

   #86505

   Purpose

   Expresses CRAF_S259A in mammalian cells

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   CRAF (RAF1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S259A

   Promoter

   CMV

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_mGFP-FGFR3-K650E

   Plasmid

   #191757

   Purpose

   Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650E mutation (associated with TD2; target mutation: c.1948A>G; silent mutation added on purpose: c.1938C>T)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc
   

   Use

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   c.1948A>G (p.K650E) + silent mutation c.1938C&…

   Promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Dakap-Venus-cpVenus-FLARE-AKAR

   Plasmid

   #123338

   Purpose

   Mitochondria-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   DAKAP-Venus-cpVenus-FLARE-AKAR
   

   Use

   Tags

   N-terminal 30 amino acids from DAKAP1, Venus, and…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNL-CEF-IgA1

   Plasmid

   #187009

   Purpose

   Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

   Depositor

   Hermann Eibel

   Article

   Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

   Insert

   Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-ALK2 K235R

   Plasmid

   #80872

   Purpose

   mammalian expression of ALK2 K235R

   Depositor

   Aristidis Moustakas

   Article

   Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

   Insert

   ALK2 (ACVR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K235R

   Promoter

   CMV

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pGlomyc-Klf4

   Plasmid

   #172870

   Purpose

   Mammalian expression vector with myc-tagged mouse Klf4 coding sequence

   Depositor

   Renee van Amerongen

   Article

   Mourao et al bioRxiv 2021.06.22.449461

   Insert

   Klf4 (Klf4 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. DN1-Gli3 in AINGpCAGGS

    Plasmid

    #121970

    Purpose

    medium Gli3 transcriptional activator

    Depositor

    James Briscoe

    Article

    Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

    Insert

    DN2Gli3 (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc

    Expression

    Mammalian

    Mutation

    N terminus deletion (nt1-864)

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPGK-T7/2-CD44s (-cyt)

    Plasmid

    #137815

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44s (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region

    Promoter

    PGK

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. KCNE2_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176980

    Purpose

    Mammalian expression vector encoding KCNE2 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    KCNE2 (KCNE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPGK-T7/2-CD44v6-10 (-cyt)

    Plasmid

    #137821

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v6-10 (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region

    Promoter

    PGK

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

    Plasmid

    #181843

    Purpose

    GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of residues 12-112 in PKA RIα.

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR3-FLAG-Gephyrin GC

    Plasmid

    #68818

    Purpose

    Flag tagged mammalian expression of GC domain

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    NIL

    Promoter

    CMV

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

    Plasmid

    #113953

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    delete N-terminal 21 residues, I132S, S212N, and …

    Promoter

    cmv

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Flag Depdc5 pLJM1

    Plasmid

    #46336

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Differs from Depdc5 isoform 1 by 10 amino acids …

    Promoter

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pLenti-CaMKIIa-SpyTag-C1C2-mCherry

    Plasmid

    #69833

    Purpose

    Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing SpyTag-C1C2-mCherry fusion. SpyTag can be used to monitor membrane localization of the C1C2 opsin.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

    Insert

    SpyTag-C1C2
    

    Use

    Lentiviral

    Tags

    Trafficking signal and mCherry

    Expression

    Mammalian

    Mutation

    Inserted SpyTag after the signal peptide of C1C2.

    Promoter

    CamKIIa

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMG058 MBP-GS 634-666-His

    Plasmid

    #154077

    Purpose

    Expresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    GYS1 (GYS1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLJM60-FLAG-SLC38A9.1 I68A

    Plasmid

    #71864

    Purpose

    lentiviral stable expression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized, Isoleucine 68 mutated to Alanine

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only