We narrowed to 16,622 results for: puromycin

1. pLib6.4-Aaeg_763

   Plasmid

   #176658

   Purpose

   Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-EGFP-Rheb-HVR HRas-IP

   Plasmid

   #13833

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

   Insert

   Rheb-HVR HRas (Rheb Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminal of Rheb and hyper variable region (HVR…

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

3. p6869 pOZ-N puro HA-HPV18 E2

   Plasmid

   #35134

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   HPV18 E2 (E2 HPV18)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   March 9, 2012

   Availability

   Academic Institutions and Nonprofits only

4. hPlekhm1-C/pIRES puro Glue (S778-A1056)

   Plasmid

   #73456

   Purpose

   Express human C-terminal part (Q778-A1056) Plekhm1 in mammalian cells

   Depositor

   Paul Odgren

   Article

   Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Tags

   CBP tag, HA tag, and strep tag

   Expression

   Mammalian

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

5. hPlekhm1-N/pIRES puro Glue (M1-A497)

   Plasmid

   #73454

   Purpose

   Express human N-terminal part (M1-A497) Plekhm1 in mammalian cells

   Depositor

   Paul Odgren

   Article

   Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Tags

   CBP tag, Ha tag, and strep tag

   Expression

   Mammalian

   Available Since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pLG1-puro-sgATL3-2

   Plasmid

   #109010

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL3-sgRNA #2 (ATL3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   mU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PPP2R5C B11.5 gRNA

   Plasmid

   #90853

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PPP2R5C

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   PPP2R5C (Guide Designation B11.5)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. TUBB F2.4 gRNA

   Plasmid

   #90925

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TUBB

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   TUBB (Guide Designation F2.4)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. p8141 MSCV-P C-FlagHA 18E7 D10K

   Plasmid

   #163306

   Purpose

   Expresses HPV18 E7 D10K

   Depositor

   Elizabeth White

   Article

   Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

   Insert

   HPV18 E7 D10K
   

   Use

   Retroviral

   Tags

   FlagHA

   Mutation

   D10K

   Promoter

   MSCV LTR

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. NUMA1 H11.2 gRNA

    Plasmid

    #90808

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUMA1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NUMA1 (Guide Designation H11.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK1224

    Plasmid

    #84221

    Purpose

    Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, BFP

    Depositor

    Martin Kampmann, Jonathan Weissman

    Article

    Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUPI

    Plasmid

    #80279

    Purpose

    A bicistronic lentiviral vector used to stably express cDNAs.

    Depositor

    Jeremy Luban

    Article

    Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

    Insert

    Puro resistance cassette, IRES, cloning site
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Non-targeting CRISPRi plasmid

    Plasmid

    #154891

    Purpose

    Human non-targeting CRISPRi gRNA

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Lalli et al Genome Res. 2020 Sep;30(9):1317-1331. do

    Insert

    Nontargeting gRNA
    

    Use

    CRISPR and Lentiviral

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. GG-EBNA-TdT-guide1-PGK-Puro

    Plasmid

    #102903

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of a control gRNA targeting TdTomato sequence. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    TdT-guide1-PGK-Puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. NUMA1 H12.2 gRNA

    Plasmid

    #90809

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUMA1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NUMA1 (Guide Designation H12.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CDC6 B5.1 gRNA

    Plasmid

    #90596

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDC6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CDC6 (Guide Designation B5.1)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. KMT5A H2.3 gRNA

    Plasmid

    #90727

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KMT5A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    KMT5A (Guide Designation H2.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. UNG D12.3 gRNA

    Plasmid

    #90937

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human UNG

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    UNG (Guide Designation D12.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PRC1 G2.2 gRNA

    Plasmid

    #90857

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRC1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PRC1 (Guide Designation G2.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PRC1 G1.2 gRNA

    Plasmid

    #90856

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRC1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PRC1 (Guide Designation G1.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge