We narrowed to 7,014 results for: tac

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pJZC40

Plasmid

#62334

Purpose

sgRNA + 2x PP7 with mCherry for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2x PP7
mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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px330-RPL26 sgRNA2

Plasmid

#134642

Purpose

contains sgRNA targeting to the C-terminus of human RPL26 for gene editing

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

RPL26 sgRNA2 (RPL26 Human)

Expression

Mammalian

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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px330-RPL26 sgRNA1

Plasmid

#134641

Purpose

contains sgRNA targeting to the C-terminus of human RPL26 for gene editing

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

RPL26 sgRNA2 (RPL26 Human)

Expression

Mammalian

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-IDS2_DF_A4b_attP_fwd

Plasmid

#182149

Purpose

To insert attP-fwd attachment site at IDS2 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

IDS2-A4b-attP-fwd pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-IDS_DF_D1b_attB_rev

Plasmid

#182152

Purpose

To insert attB-rev attachment site at IDS in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

IDS-D1b-attB-rev pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-IDS_DF_C2c_attB_rev

Plasmid

#182151

Purpose

To insert attB-rev attachment site at IDS in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

IDS-C2c-attBrev pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-IDS2_DF_B7b_attP_fwd

Plasmid

#182150

Purpose

To install attP-fwd attachment site at IDS2 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

IDS2-B7b-attP-fwd pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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MPC11 IgG2b gene

Plasmid

#127248

Purpose

Expresses intact IgG2b gene in mammalian cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

IgG2b gene with exons and alt pAs (Ighg2b Mouse)

Expression

Mammalian

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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gh172

Plasmid

#106833

Purpose

expression of gRNA targeting UGGT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

UGGT1 (UGGT1 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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TET-pLKO-neo CXCR4-sh1

Plasmid

#163741

Purpose

Lentivirus for inducible knockdown of CXCR4 (human)

Depositor

Roland Friedel

Insert

CXCR4 (CXCR4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

TRE

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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psbA2(noATbox)-PHLS (c)

Plasmid

#52308

Purpose

Replaces the psbA2 gene in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Mutation

CAAATACA box in the psbA2 promoter to ggcgcgcc

Promoter

psbA2

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pPAct2noUTR (GB0192)

Plasmid

#68177

Purpose

Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtAct2 promoter (no UTR)

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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YFP(159-238) in pcDNAI/Amp

Plasmid

#60889

Purpose

This vector attaches YFP(159-238) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

Insert

YFP(159-238)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-malG

Plasmid

#89951

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

malG gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pPN144

Plasmid

#91673

Purpose

Express sgRNA targeting human TBC1D5

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

TBC1D5 (TBC1D5 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN145

Plasmid

#91674

Purpose

Express sgRNA targeting human TBC1D5

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

TBC1D5 (TBC1D5 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN023

Plasmid

#91671

Purpose

Express sgRNA targeting human SYNGAP1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SYNGAP1 (SYNGAP1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLVTHM-shREV3-4

Plasmid

#38033

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

Insert

shREV3-4 (Rev3l, Rev3l, REV3L Mouse, Rat, human, Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
gh242

Plasmid

#106850

Purpose

expression of gRNA targeting PIGA

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

PIGA (PIGA Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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gh174

Plasmid

#106835

Purpose

expression of gRNA targeting UGT8

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

UGT8 (UGT8 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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