We narrowed to 7,493 results for: addgene/1000

Showing: 5061 - 5080 of 7493 results

Results per page:

Sort results by:

pCMV-TAPtagN-PrP-SBP-HsAGO2_AC

Plasmid

#148440

Purpose

Mammalian Expression of HsAGO2

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsAGO2 (AGO2 Human)

Expression

Mammalian

Mutation

one silent mutation C840Tand sequence differs in …

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-mCherry-SynaptoZip

Plasmid

#177318

Purpose

AAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoter

Depositor

Antonio Malgaroli

Article

Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

Insert

mCherry-SynaptoZip (Vamp2 Rat)

Use

AAV

Tags

Myc and mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJET-22-DExCon-mNeonGreen-R11a

Plasmid

#179897

Purpose

Donor with homologous arms for Rab11a to knock in DExCon-mNeonGreen module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11A, member RAS oncogene family (RAB11A Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pspCas9-3exo-Tetra-com-CLCN5-sp-g1

Plasmid

#176236

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176237

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pspCas9-Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176238

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PWPXLD-EGFR-P667A

Plasmid

#133750

Purpose

EGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid

Depositor

Chay Kuo

Article

Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

Insert

Mutant P667A EGFR-HA (EGFR Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

proline 667 converted to alanine (Please see depo…

Promoter

EF1-alpha

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28B-PU.1-ETS-H3

Plasmid

#124435

Purpose

Chimeric ETS domain: H3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis Tag

Expression

Bacterial

Mutation

Residues 225 to 239 replaced with corresponding s…

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28B-PU.1-ETS-Loop

Plasmid

#124551

Purpose

Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 216 to 223 replaced with corresponding s…

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28B-PU.1-ETS-S3

Plasmid

#124550

Purpose

Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 242 to 246 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-PU.1-ETS-H2

Plasmid

#123358

Purpose

Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 207 to 215 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLC-Flag-CSNK1A1 G40N-P2A-Blast

Plasmid

#123321

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Blast casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

changed Glycine 40 to Asparagine

Promoter

CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-CD4-YEML-myc

Plasmid

#58538

Purpose

Expresses human CD4 with the IFITM3 endocytosis motif (YEML) and a C-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

CD4 (CD4 Human)

Tags

IFITM3 endocytosis Tyrosine-Glutamate-Methionine-…

Expression

Mammalian

Mutation

Added C-terminal Tyrosine-Glutamate-Methionine-Le…

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2-Cx43-M100/125/147L-siResist

Plasmid

#49856

Purpose

Encodes human connexin 43 with M100, M125, and M147 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100, Methionine 125, and Methionine 147…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2-Cx43-M100/125L-siResist

Plasmid

#49855

Purpose

Encodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100 and Methionine 125 mutated to Leuci…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC

Plasmid

#69585

Purpose

Expresses mutated p53-L344A tagged with mKate2 and split C-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53-L344A (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - C term

Expression

Mammalian

Mutation

p53 L344A mutation that prevents tetramerization …

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_SFFv_Her2-BFP_RHL027

Plasmid

#164823

Purpose

Lentiviral vector for constitutive expression of HER2+TM(23-675)-BFP

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Insert

Extracellular and transmembrane region of ERBB2 (ERBB2 Human)

Use

Lentiviral

Tags

tagBFP

Promoter

SFFV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF2941

Plasmid

#144417

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

DLX5 (DLX5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZac2.1-GfaABC1D-Ezrin T567A-BioID2-HA

Plasmid

#227683

Purpose

To over express Phospho-dead Ezrin under gfaABC1D promoter in astrocytes

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

Ezrin (Ezr Mouse)

Tags

BioID2-HA

Expression

Mammalian

Mutation

T567A

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-InPGrp-Kras

Plasmid

#236001

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to plasma membrane.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

InPGrp-Kras (Cyth3 Rat, Synthetic)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge