We narrowed to 16,579 results for: grna

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pAAV-U6-CB2gRNA-CBh-mCherry

Plasmid

#91948

Purpose

Expression of gRNA for mouse CB2 cannabinoid receptor and mCherry

Depositor

Jimok Kim

Article

Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

Insert

mCherry

Use

AAV

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pS23.U6<ActB.mus_C-term-KI_gRNA

Plasmid

#207408

Purpose

gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

Depositor

Alexandros Poulopoulos

Article

Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

Insert

ActB

Use

CRISPR and Mouse Targeting

Promoter

U6

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSico_U6-Pan-PGC1a sgRNA

Plasmid

#165426

Purpose

Expression of gRNA against human Total PGC-1a variants

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

gRNA against human Total PGC-1a variants (PPARGC1A Human, Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

Plasmid

#149588

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nelfb-g1)-PGKpuroBFP-W

Plasmid

#105023

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfb (Cobra1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nelfb-g2)-PGKpuroBFP-W

Plasmid

#105024

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfb (Cobra1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W

Plasmid

#105025

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfcd (Th1l Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-W

Plasmid

#105026

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfcd (Th1l Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-U63-tgRNA-nlsBFP

Plasmid

#169029

Purpose

gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

Depositor

Chun Han

Article

Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC12)_Psyn-sgRNArodA

Plasmid

#149661

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC12), Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pX601‐mhCMV‐ABEmaxNGA‐C3‐ E53ogRNA

Plasmid

#187063

Purpose

Npu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

Npu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mhCMV

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNAftsI

Plasmid

#149590

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pM116: CasRx VEGFA presgRNA

Plasmid

#166868

Purpose

U6-driven expression of human VEGFA targeting presgRNA compatible with CasRx.

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Insert

Human VEGFA targeting presgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-SpCas9_sgRNA-site2 (RTW448)

Plasmid

#160137

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-scramble

Plasmid

#62285

Purpose

expression of scramble sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

scramble sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [n] (GB1207)

Plasmid

#75408

Purpose

tRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [n]

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCas9-Puro HLAG-2B-sgRNA

Plasmid

#182552

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Article

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2B-sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA SOX17 -91

Plasmid

#50923

Purpose

U6 driven sgRNA targeting Sox17 -91 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Sox17 -91 sgRNA (SOX17 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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gRNAs[bTub+Sxl].1046B

Plasmid

#112692

Purpose

express two gRNA targeting bTub & Sxl under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP

Plasmid

#89061

Purpose

AAV-gRNA targeting the murine Ldlr gene

Depositor

William Lagor

Article

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

Chicken beta actin with partial CMV enhancer

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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