We narrowed to 30,806 results for: Ide

1. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

   Plasmid

   #175573

   Purpose

   Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CROPseq-EFS-HypaSpCas9-P2A-puro

   Plasmid

   #106965

   Purpose

   single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

   Depositor

   Alex Hewitt

   Insert

   HypaSpCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBolux

   Plasmid

   #188109

   Purpose

   Encodes a promoter-less Bacteroides-optimized lux cassette

   Depositor

   Guy Townsend

   Article

   Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

   Plasmid

   #175574

   Purpose

   Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOpen-GBDpol (exo)

   Plasmid

   #165572

   Purpose

   Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
   

   Use

   Synthetic Biology

   Available Since

   Aug. 24, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGEN-MCS

   Plasmid

   #44919

   Depositor

   Harry Mobley

   Article

   Lane et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

   Type

   Empty backbone

   Use

   Stable complementation

   Promoter

   none

   Available Since

   May 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWT015a

   Plasmid

   #96878

   Purpose

   GFP reporter

   Depositor

   David Liu

   Article

   Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

   Insert

   GFP
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV hSyn GFP-Cre

   Plasmid

   #175381

   Purpose

   expresses GFP-Cre under the control of a human synapsin promoter

   Depositor

   Jason Aoto

   Insert

   GFP-Cre recombinase
   

   Use

   AAV

   Tags

   Cre is fused to GFP. GFP is fused to additional n…

   Promoter

   human synapsin

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIS2

   Plasmid

   #12177

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. NPY-GFP

    Plasmid

    #74629

    Purpose

    human neuropeptide Y fused to GFP

    Depositor

    Justin Taraska

    Insert

    Neuropeptide Y (NPY Human)
    

    Tags

    mEGFP

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB33eCPX

    Plasmid

    #23336

    Depositor

    Patrick Daugherty

    Article

    Rice et al Protein Eng Des Sel. 2008 Jul . 21(7):43

    Insert

    Enhanced Circularly Permuted Outer Membrane Protein X
    

    Expression

    Bacterial

    Available Since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKNOCK-Km

    Plasmid

    #46262

    Purpose

    Bacterial targeting

    Depositor

    Mikhail Alexeyev

    Article

    Alexeyev et al Biotechniques. 1999 May;26(5):824-6, 828

    Type

    Empty backbone

    Use

    Bacterial targeting

    Available Since

    Aug. 14, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pBiFC-CrN173

    Plasmid

    #22016

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    CrN173
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBiFC-CC155

    Plasmid

    #22015

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    CC155
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKNOCK-Gm

    Plasmid

    #46260

    Purpose

    Bacterial targeting

    Depositor

    Mikhail Alexeyev

    Article

    Alexeyev et al Biotechniques. 1999 May;26(5):824-6, 828

    Type

    Empty backbone

    Use

    Bacterial targeting

    Available Since

    Aug. 14, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pKNOCK-Tc

    Plasmid

    #46259

    Purpose

    Bacterial targeting

    Depositor

    Mikhail Alexeyev

    Article

    Alexeyev et al Biotechniques. 1999 May;26(5):824-6, 828

    Type

    Empty backbone

    Use

    Bacterial targeting

    Available Since

    Aug. 14, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCSC-SP-PW-GFP (aka: pBOB-GFP)

    Plasmid

    #12337

    Depositor

    Inder Verma

    Article

    Marr et al J Mol Neurosci. 2004 . 22(1-2):5-11.

    Insert

    GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBJ5 mCnB

    Plasmid

    #17872

    Depositor

    Jerry Crabtree

    Article

    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

    Insert

    CnB (Ppp3r1 Mouse)
    

    Expression

    Mammalian

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEntr-Tbx5flbio

    Plasmid

    #32968

    Depositor

    William Pu

    Article

    He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

    Insert

    Tbx5 (TBX5 Human)
    

    Use

    Gateway

    Tags

    flag-bio

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSG06-pLac-NRI

    Plasmid

    #102463

    Purpose

    Expresses NRI protein in E. coli. The expression is controlled by a pLac promoter.

    Depositor

    Richard Murray

    Article

    Guo et al bioRxiv 122606

    Insert

    NRI (ntrC )
    

    Expression

    Bacterial

    Promoter

    pLac

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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