We narrowed to 19,027 results for: Tec;

1. pFLIPglu-3.2mDelta13

   Plasmid

   #12992

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

   Insert

   FLIPglu F16A S112A Delta13
   

   Use

   Plant nanosensor

   Tags

   ECFP and EYFP

   Expression

   Plant

   Mutation

   F16A, S112A in binding protein

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

   Plasmid

   #87560

   Purpose

   Expresses aa397-423 of Flp recombinase fused to PYL CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   FlpO aa397-423
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   PYL

   Expression

   Mammalian

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

   Plasmid

   #87559

   Purpose

   Expresses aa1-396 of Flp recombinase fused to ABI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   FlpO aa1-396
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   ABI

   Expression

   Mammalian

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEAST2-sfGFP

   Plasmid

   #126575

   Purpose

   Strong constitutive expression of super-folder GFP for cell-free expression

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p413GAL1-Zipp.-L-IFP-F[2] (Yeast)

   Plasmid

   #52893

   Purpose

   Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Zipp.-L-IFP-F[2]
   

   Tags

   L-IFP-F[2]

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pMM545-Tier1-PhCMV-Citrine

   Plasmid

   #169505

   Purpose

   Tier-1 vector encoding PhCMV-driven yellow fluorescent protein Citrine (PhCMV-Citrine-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven yellow fluorescent protein citrine
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCellFree_G03 PRKCA

   Plasmid

   #67115

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PRKCA Protein kinase C alpha type (PRKCA Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. PB-HD118

   Plasmid

   #172655

   Purpose

   Expressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 gene

   Depositor

   Jay Shendure

   Article

   Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   pegRNA-HD118A/pegRNA-HD118B
   

   Use

   CRISPR

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFLIPglu-170nDelta13

   Plasmid

   #12989

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

   Insert

   FLIPglu F16F Delta13
   

   Use

   Plant nanosensor

   Tags

   ECFP and EYFP

   Expression

   Plant

   Mutation

   WT binding protein (no F16A)

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

10. TAL2406

    Plasmid

    #36809

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    JAK2-TALEN_Left (Jak2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. TAL2237

    Plasmid

    #36688

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    CHD4-TALEN_Right (Chd4 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. LLP762_L2pV1_OR-OCS_TCTP-Flp_s35S-Cre

    Plasmid

    #192421

    Purpose

    To act as the 2-input state of the OR gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-loxP-OCS-FRT-loxP::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mTFP1-Actin-7

    Plasmid

    #55462

    Purpose

    Localization: Actin, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

    Insert

    Actin (ACTB Human)
    

    Tags

    mTFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pET15b+PCNA1 WT

    Plasmid

    #66139

    Purpose

    Expresses the wild type of Sulfolobus solfataricus PCNA1

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 1
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. TAL2407

    Plasmid

    #36810

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    JAK2-TALEN_Right (Jak2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

16. EK0499 SFFV-mCherry-SGc(PPIB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191183

    Purpose

    Expression of PPIB sensor in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:PPIB.s1:EGFP-3xMS2
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB6679

    Plasmid

    #106158

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6638, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. SEPT11 sh1 shRNA

    Plasmid

    #122020

    Purpose

    Knock Down of Septin 11. It targetes Septin 11 mRNA. It knocks down all known splice variants of Sept 11 mRNAs.

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 shRNA (Sept11 Rat)
    

    Use

    RNAi

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-KG/Net1

    Plasmid

    #28260

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Net1 (Net1 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 11, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pBM13-sd4U

    Plasmid

    #179691

    Purpose

    Bacterial expression of base editor

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [sd4U] UGI-TALE3-DddA-G1397-N (Q1310R + S1330I + T1380I)
    

    Expression

    Bacterial

    Mutation

    Q1310R, S1330I, T1380I

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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