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Showing: 7681 - 7710 of 14826 results

  4. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid
    #181791
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181791-plasmid-map-sequence-id-354814

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  10. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    70707-plasmid-map-sequence-id-127770

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  15. pIMVAu1

    Plasmid
    #98301
    Purpose
    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.
    Depositor
    Claudia Vickers
    Article
    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.
    Insert
    HMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    98301-plasmid-map-sequence-id-190182

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  20. pcg 2.0-BFP-circ-arRNA backbone

    Plasmid
    #213965
    Purpose
    pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o
    Depositor
    Wensheng Wei
    Article
    Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    213965-plasmid-map-sequence-id-422887

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  21. pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

    Plasmid
    #181790
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181790-plasmid-map-sequence-id-354811

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  22. xCas9

    Plasmid
    #138565
    Purpose
    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    xCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138565-plasmid-map-sequence-id-274797

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  24. pAAV-CaMKII-STChRger2-TS-EYFP

    Plasmid
    #129393
    Purpose
    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    soma targeted ChRger2
    Use
    AAV
    Tags
    Kv2.1-TS-EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    129393-plasmid-map-sequence-id-258245

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  26. pBbE1k-3

    Plasmid
    #71722
    Purpose
    tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human
    Depositor
    Taek Soon Lee
    Article
    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1
    Inserts
    dihydropterine reductase (QDPR Human)
    pterin-4a-carbinolamine dehydratase (PCBD1 Human)
    Use
    Tags
    Expression
    Mutation
    Promoter
    pTrc
    Available since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    71722-plasmid-map-sequence-id-129984

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Showing: 7681 - 7710 of 14826 results