We narrowed to 59,994 results for: %s

1. REV human OCT4 TALEN

   Plasmid

   #52413

   Purpose

   TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Insert

   HD HD HD HD HD NI NG NG HD HD NG NI NN NI NI NN NN
   

   Use

   TALEN

   Promoter

   CAGGS

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCW57.1-FRB-MCU-Flag-TetOFF

   Plasmid

   #185659

   Purpose

   Tet/dox-repressible expression of N-terminus FRB tagged human mitochondrial calcium uniporter with C-terminus Flag epitope

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   Mitochondrial calcium uniporter (MCU Human)
   

   Use

   Lentiviral

   Tags

   FRB and Flag

   Expression

   Mammalian

   Promoter

   pTight TRE

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pINTO-N3::hTET3 V908L

   Plasmid

   #136370

   Purpose

   Expresses epitope-tagged human TET3 V908L variant in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Tags

   2xStrep-tagII, FLAG, and HA

   Expression

   Mammalian

   Mutation

   V908L

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pINTO-N3::hTET3 V1089M

   Plasmid

   #136369

   Purpose

   Expresses epitope-tagged human TET3 V1089M variant in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Tags

   2x Strep-tagII, FLAG, and HA

   Expression

   Mammalian

   Mutation

   V1089M

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pINTO-N3::hTET3 A1076T

   Plasmid

   #136368

   Purpose

   Expresses epitope-tagged human TET3 A1076C variant in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Tags

   2xStrepTagII, FLAG, and HA

   Expression

   Mammalian

   Mutation

   A1076T

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINTO-N3::hTET3 R752C

   Plasmid

   #136367

   Purpose

   Expresses epitope-tagged human TET3 R752C variant in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Tags

   2xStrepTagII, FLAG, and HA

   Expression

   Mammalian

   Mutation

   R752C

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pINTO-N3::hTET3 F1072C

   Plasmid

   #136366

   Purpose

   Expresses epitope-tagged human TET3 F1072C variant in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Tags

   2xStrepTagII, FLAG, and HA

   Expression

   Mammalian

   Mutation

   F1072C

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. HSPB1

   Plasmid

   #155670

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   HSPB1 (HSPB1 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-hSyn-GRAB_ACh3.0

   Plasmid

   #121922

   Purpose

   express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

   Depositor

   Yulong Li

   Article

   Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

   Has Service

   AAV9

   Insert

   GPCR activation based ACh sensor GRAB-ACh3.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiCRISPRv2 blast

    Plasmid

    #98293

    Purpose

    Variant of lentiCRISPRv2 that confers blasticidin S resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF-1a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-EF1a-iCreV

    Plasmid

    #140135

    Purpose

    Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Has Service

    AAV PHP.eB and AAV1

    Insert

    iCreV
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GAPDH

    Plasmid

    #155622

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    GAPDH (GAPDH Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-SpCas9 blast

    Plasmid

    #104997

    Purpose

    This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX_TRC311/NLS-Cas13d-P2A-Blast

    Plasmid

    #212196

    Purpose

    CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40

    Depositor

    Stacey Edwards

    Article

    Wang et al Mol Cancer. 2024 May 15;23(1):101. doi:

    Insert

    CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
    

    Use

    CRISPR and Lentiviral

    Tags

    HA, NLS, and blasticidin S deaminase

    Expression

    Mammalian

    Promoter

    EF-1α promoter

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-iFlpV

    Plasmid

    #140137

    Purpose

    can be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinase

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Has Service

    AAV PHP.eB and AAV1

    Insert

    iFlpV
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-iDreV

    Plasmid

    #140136

    Purpose

    can be used to generate AAV virus that will express light-inducible site-specific iDreV recombinase

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Has Service

    AAV PHP.eB and AAV1

    Insert

    iDreV
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Td-CGBE

    Plasmid

    #196599

    Purpose

    Mammalian expression of Td-CGBE

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
    

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    TadA-8e (N46L); S. pyogenes Cas9 (D10A)

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-DIO-GRAB_ACh3.0

    Plasmid

    #121923

    Purpose

    express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

    Insert

    GPCR activation based ACh sensor GRAB-ACh3.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-EF1a-CCreV

    Plasmid

    #140132

    Purpose

    can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Insert

    CCreV
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-EF1a-NCreV

    Plasmid

    #140131

    Purpose

    can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Insert

    NCreV
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge