We narrowed to 33,007 results for: FER

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TetO-FUW-NfiA

Plasmid

#64901

Purpose

Inducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.

Depositor

Vania Broccoli

Article

Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

Insert

Nfia (Nfia Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_dC_mKate2

Plasmid

#167336

Purpose

Expresses mKatet2 fused to TFAM dC-tail mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) as well a…

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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WN10150

Plasmid

#80443

Purpose

Expresses dead (908A) AsCpf1

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

nuclease dead (908A) AsCpf1

Tags

NLS-3xHA

Expression

Mammalian

Mutation

nuclease dead (D908A)

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 myc-Rab10

Plasmid

#208366

Purpose

Mammalian expression of myc-tagged human Rab10

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

myc-Rab10 (RAB10 Human)

Expression

Mammalian

Mutation

wild type

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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SID4x-dCas9-KRAB

Plasmid

#106399

Purpose

Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain

Depositor

Jason Gertz

Article

Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

Inserts

SID4x (MXD1 Human)
KRAB (ZNF10 Human)

Tags

FLAG, HA, and NLS

Expression

Mammalian

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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PGL4-4X Mut Renilla

Plasmid

#70040

Purpose

Control plasmid for testing 5'UTR folding elements

Depositor

Hans-Guido Wendel

Article

Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

Insert

Random sequence matched for GC-content

Use

Luciferase

Tags

renilla luciferase

Expression

Mammalian

Promoter

SV40

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
Polymerase expression - pCMV-T7-EcKlenow (LM2692)

Plasmid

#208963

Purpose

Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

EcKlenow-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

Plasmid

#114103

Purpose

fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi

Insert

sbGLuc-VChR1-EYFP

Use

AAV

Expression

Mammalian

Promoter

motoneuron-specific Homeobox 9 promoter

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLL7 lenti-dcas9-3xflag-blast

Plasmid

#112133

Purpose

lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

dCas9(D10A, N863A)-T2A-Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A mutants in Cas9

Promoter

EF1a

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFlag IFIT2

Plasmid

#53555

Purpose

mammalian expression of IFIT2

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT2 (IFIT2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pOTTC385 - pAAV CMV-IE IRES EGFP

Plasmid

#102936

Purpose

An AAV vector that expresses IRES EGFP under the CMV-IE promoter

Depositor

Brandon Harvey

Article

Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

CMV-IE

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-L647RLaminA

Plasmid

#69060

Purpose

encodes a lamin A mutant with Leucine 647 mutated to an Arginin

Depositor

Gerardo Ferbeyre

Article

Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

Insert

LaminA (LMNA Human)

Use

Retroviral

Expression

Mammalian

Mutation

Lamin A with Leucine 647 mutated to Arginine

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only
pGEX:PGRMC1

Plasmid

#136062

Purpose

Bacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.

Depositor

Francisco Domínguez Hernández

Article

Salsano et al Fertil Steril. 2020 May;113(5):1050-1066

Insert

Progesterone receptor membrane component 1 (PGRMC1 Human)

Tags

GST-PGRMC1

Expression

Bacterial

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-2xPH-TAPP1 R211L mutant

Plasmid

#161991

Purpose

Expresses two mutated tandem repeats of Tapp1 PH domain (phosphoinositide binding-deficient mutant of TAPP1, R211L, can't bind PI(3,4)P2). Fused to eGFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PH-TAPP1 (PLEKHA1 Human)

Expression

Mammalian

Mutation

R211L

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLPC-S22A-LaminA

Plasmid

#69066

Purpose

encodes a mutant LaminA-CS where serine 22 is mutated to an alanine

Depositor

Gerardo Ferbeyre

Article

Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

Insert

LaminA-CS (LMNA Human)

Use

Retroviral

Expression

Mammalian

Mutation

serine 22 mutated in alanine

Promoter

CMV

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
EXT1- D164A-pLJC2-3XFLAG

Plasmid

#215828

Purpose

Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 Mutant (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS4-dTom

Plasmid

#178718

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

dTom

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-pro-alphaf(I)-E2-Crimson

Plasmid

#117660

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-pro-alphaf(I)-E2-Crimson

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGFP-HBO1-G485A pc2201

Plasmid

#167570

Purpose

The human HBO1 is fused to C-terminal GFP of EGFP-C1 (Clontech)

Depositor

Cristina Cardoso

Article

Heinz et al Epigenetics Chromatin. 2019 Mar 14;12(1)

Insert

histone acetyltransferase 1 (KAT7 Human)

Tags

GFP

Expression

Mammalian

Mutation

G485A

Promoter

CMV

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET32a-hHBEGF

Plasmid

#199234

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Proheparin-binding EGF-like growth factor (HBEGF Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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