We narrowed to 31,702 results for: LIS;

1. IAA17-4flag-HIS5

   Plasmid

   #207015

   Purpose

   For ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ClhN-HIS5

   Plasmid

   #207014

   Purpose

   Plasmid backbone for yeast expression, use auxotrophic marker HIS5 (Schizosaccharomyces Pombe)

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ClhN-MET15

   Plasmid

   #207013

   Purpose

   Plasmid backbone for yeast expression, use auxotrophic marker MET15 (Saccharomyces cerevisiae)

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ClhN-URA3

   Plasmid

   #207012

   Purpose

   Plasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis)

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3'-BirA*-FLAG minimal CMV pCDNA5

   Plasmid

   #212047

   Purpose

   LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. 5'-BirA*-FLAG minimal CMV pCDNA5

   Plasmid

   #212046

   Purpose

   LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 5'-3xFLAG GFP minimal CMV pCDNA5

   Plasmid

   #212086

   Purpose

   LR vector for integration of GFP into N2a FRT rtTA3 expression cells

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   GFP
   

   Expression

   Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. GB1-His-Tev-ALIX-V (348-702)

   Plasmid

   #189819

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His-Tev-ALIX-V (348-702).

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   GB1-His-Tev-ALIX-V (348-702) (PDCD6IP Human)
   

   Tags

   GB1-His-Tev

   Expression

   Bacterial

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b I-SceI (Ctermhis) K223I

   Plasmid

   #195615

   Purpose

   Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Expression

   Bacterial

   Mutation

   C2694G, G2345C, G2351C, A3365T, A3366T

   Promoter

   T7

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15b I-SceI (Ctermhis) K122I

    Plasmid

    #195614

    Purpose

    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity

    Depositor

    Frederick Gimble

    Article

    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

    Insert

    I-SceI
    

    Expression

    Bacterial

    Mutation

    C2458G, A2826T, A2827T

    Promoter

    T7

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPG-GPD-TRPC

    Plasmid

    #190113

    Purpose

    Expression vector for fungal (Aspergillus flavus) genes based on Aspergillus parasiticus pyrG gene selection

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Fungal Genet Biol. 2017 Jul;104:29-37. d

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBSKΔB-24xopto-TetO-no01

    Plasmid

    #174887

    Purpose

    Plasmid vector containing southern blot probe for opto-TetO in STREAMING-tag

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    24xopto-TetO
    

    Use

    Southern blot probe

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. eSpCas9-EF

    Plasmid

    #177267

    Purpose

    CRISPR vector

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLY227

    Plasmid

    #184148

    Purpose

    short crRNA generator with spacer LEA2 with WT repeat region

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-crRNA-LEA2-WT
    

    Use

    Synthetic Biology

    Promoter

    Plux2

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLY232

    Plasmid

    #184149

    Purpose

    short tracrRNA generator with WT anti-repeat region

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-tracrRNA-WT
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    araBAD promoter

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLY171

    Plasmid

    #184145

    Purpose

    sgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRa

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    sgRNA-LEA2-ex3
    

    Use

    Synthetic Biology

    Promoter

    Plux2

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLY283

    Plasmid

    #184156

    Purpose

    Reprogrammed short tracrRNA for hijacking mRNA of zntA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-tracrRNA-zntA-1034
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    araBAD promoter

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ265N1

    Plasmid

    #173998

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    rAPOBEC1-zCas9(D10A)-UNG
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    D10A

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ265O1

    Plasmid

    #173999

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    rAPOBEC1-zCas9(D10A)-rXRCC1
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    D10A

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. FnRPR9-10

    Plasmid

    #182568

    Purpose

    Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 9-10
    

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1374A, P1376A, R1379A

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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