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pRD411

Plasmid

#178196

Purpose

sRok overexpression

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

srok

Expression

Bacterial

Promoter

T7

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRD231

Plasmid

#178195

Purpose

Rok overexpression

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

rok

Expression

Bacterial

Promoter

T7

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCK411.RR1

Plasmid

#192644

Purpose

Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

BBa_J23119-RR1

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pIALD2S

Plasmid

#185865

Purpose

Knocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

Insert

ALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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KBB439

Plasmid

#185093

Purpose

NMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

GAL1::NMA111-GFP

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB280

Plasmid

#185073

Purpose

Larger fusion of Nma111 nuclear localization signal to GFP

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

NMA111 aa 1-83

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB282

Plasmid

#185074

Purpose

Smaller fusion of Nma111 nuclear localization signal to GFP

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

NMA111 aa 1-35

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB465

Plasmid

#185098

Purpose

NMA111-GFP with NLS1 deleted under endogenous promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

NMA111DNLS1-GFP

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB462

Plasmid

#185095

Purpose

NMA111-GFP with NLS1 and NLS2 mutated under control of GAL1 promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

GAL1::nma111Dnls1Dnls2-GFP

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB466

Plasmid

#185099

Purpose

NMA111-GFP with NLS1 and NLS2 deleted under endogenous promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

NMA111DNLS1DNLS2-GFP

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB467

Plasmid

#185100

Purpose

NMA111-GFP with NLS2 deleted under endogenous promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

NMA111DNLS2-GFP

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHBT-Mb(GDx-Clo_L10)

Plasmid

#183406

Purpose

Expresses Monobody GDx-Clo_L10 in E. coli

Depositor

Shohei Koide

Article

Kermani et al Nat Commun. 2020 Nov 27;11(1):6064. doi:

Insert

His tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)

Tags

His tag-Avi tag-TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDD2711

Plasmid

#175083

Purpose

Mammalian expression of Flag-tagged KSHV ORF45 carrying E223A-S226A/RC mutations

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

E223A, S226A, RC mutations

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDD2712

Plasmid

#175084

Purpose

Mammalian expression of Flag tagged KSHV ORF45 carrying E223A-S226A/RN2 mutations

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

E223A, S226A, RN2 mutations

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15484_HF-ABE8e

Plasmid

#174122

Purpose

Expresses ABE8e fused with SpCas9-HF1

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

HF-ABE8e

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNArodA

Plasmid

#149658

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9992-HF-ABE

Plasmid

#163000

Purpose

plasmid expressing HF-ABE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

HF-ABE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9994-HypaR661A-ABE

Plasmid

#163002

Purpose

plasmid expressing HypaR661A-ABE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

Hypa-ABE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Mutation

R661A

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9996-HeF-ABE

Plasmid

#163004

Purpose

plasmid expressing HeF-ABE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

HeF-ABE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRS426-CeLACE-1

Plasmid

#174523

Purpose

Express C.elegans LACE-1 in yeast cells

Depositor

Oleh Khalimonchuk

Article

Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

Insert

lace-1 (C30F12.2 Nematode)

Tags

6xHis

Expression

Yeast

Promoter

MET25

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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