We narrowed to 24,030 results for: CHI

1. pAAV_hSyn1_AchLightR

   Plasmid

   #187463

   Purpose

   Expresses red acetylcholine indicator AchLightR in neuronal cells

   Depositor

   Tommaso Patriarchi

   Article

   Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

   Insert

   AchLightR
   

   Use

   AAV

   Tags

   Igκ leader

   Expression

   Mutation

   Promoter

   human Synapsin-1

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-CAG-3xFLAG::Flcn-pgk-hph

   Plasmid

   #48757

   Purpose

   PiggyBac backbone, CAG promoter driving murine 3xFLAG-Flcn fusion, Hygromycin selection

   Depositor

   Austin Smith

   Article

   Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

   Insert

   3xFLAG::Flcn (Flcn Mouse)
   

   Use

   Piggybac

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pDONR221-MICU1-mEF

   Plasmid

   #31765

   Depositor

   Vamsi Mootha

   Article

   Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

   Insert

   MICU1 (MICU1 Human)
   

   Use

   Pdonr gateway

   Tags

   None

   Expression

   Mutation

   Mutated in both EF-hand domains: D231A, E242K, D4…

   Promoter

   Available since

   May 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-E6AP Hect

   Plasmid

   #165102

   Purpose

   bacterial expression of a GST fusion of Human E6AP Hect domain

   Depositor

   Arthur Haas

   Article

   Ronchi et al Methods Mol Biol. 2012;832:197-218. doi:

   Insert

   E6AP Hect (UBE3A Human)
   

   Use

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   E6AP Hect domain Amino acids 495-738 (5'QLNP…

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-ACBEmax

   Plasmid

   #139106

   Purpose

   Mammalian ACBEmax expression vector (pSI-632)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGIPz-PD-L1 4NQ

   Plasmid

   #121487

   Purpose

   Expression of human PD-L1 4NQ with PD-L1 shRNA

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Inserts

   PD-L1 4NQ
   hPD-L1 shRNA
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N35Q, N192Q, N200Q and N219Q

   Promoter

   CMV

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. USPL1cat-pETM30

   Plasmid

   #85768

   Purpose

   bacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusion

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, catalytic domain (aa 212-514) (USPL1 Human)
   

   Use

   Tags

   GST and His

   Expression

   Bacterial

   Mutation

   catalytic domain (aa 212-514)

   Promoter

   T7-lac

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBa.GFP-KIF13A tail 361-1749

   Plasmid

   #134622

   Purpose

   Mammalian expression of mouse KIF13A 361-1749

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF13A (Kif13a Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 360 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963

   Plasmid

   #134612

   Purpose

   Mammalian expression of mouse KIF5B 376-963

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF5B (Kif5b Mouse)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   WT with deletion of the 375 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-hPCM2w/b delta6

    Plasmid

    #47588

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2w/b delta6 (ATP2B2 Human)
    

    Use

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Mutation

    6 C-terminal AA deleted (needed for PDZ binding)

    Promoter

    CMV

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR P2R-P3-EGFP

    Plasmid

    #48349

    Purpose

    Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Aminoacid 40 is valine in our sequence, while it …

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBa.GFP-KIF13B tail 442-1843

    Plasmid

    #134623

    Purpose

    Mammalian expression of mouse KIF13B 442-1843

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF13B (Kif13b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 441 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBiFC-HA-hCaspase 2 FL (C303S)-VC155

    Plasmid

    #49257

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 (CASP2 Human)
    

    Use

    Tags

    HA and split Venus C

    Expression

    Mammalian

    Mutation

    C303S

    Promoter

    CMV

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

14. Sec61 beta pAcGFP-C1 (802)

    Plasmid

    #62008

    Purpose

    mammalian expression of GFP-Sec61 beta

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    Sec61 beta (SEC61B Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA-PU.1-ETS

    Plasmid

    #124652

    Purpose

    Chimeric ETS domain

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. gZiPro

    Plasmid

    #198441

    Purpose

    Activity/inhibition assays, 19F-NMR

    Depositor

    Tanja Schirmeister, Ute Hellmich

    Article

    Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

    Insert

    glycine linked Zika virus NS2B-NS3
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    R95*A,

    Promoter

    T7 promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-dLight1.2

    Plasmid

    #111069

    Purpose

    To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV1

    Insert

    dLight1.2
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR P2R-P3-V5-6xHis

    Plasmid

    #48347

    Purpose

    Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    V5 and 6xHis epitope tags cassette
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Contains a stop codon at the end of the epitope t…

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. USPL1cat-pEYFP_C1

    Plasmid

    #85766

    Purpose

    mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, catalytic domain (aa 212-514) (USPL1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    catalytic domain (aa 212-514)

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDESTpK7WG-RPS5Ap-mito

    Plasmid

    #163145

    Purpose

    Plant mitoTALEN vector assembly, Destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the RPS5A promoter

    Depositor

    Shin-ichi Arimura

    Article

    Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1

    Insert

    RPS5A promoter and mitochondrial presequence in front of attR1 in pk7WG
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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