We narrowed to 16,245 results for: LEA
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Plasmid#120237PurposeExpresses V5-tagged Smpd4 in lentiviral vectorDepositorAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only
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SuExp-mPLD3
Plasmid#201245Purposeexpress full length mouse PLD3 proteinDepositorAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Endosomal Escape Control
Plasmid#186552PurposeNegative Control confirming the need for a CPP peptide in mediating internalizationDepositorInsert6H-ETA-GFP
Tags6xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization and Endosomal Escape
Plasmid#186551PurposeInternalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cellsDepositorInsert10R-8His-ETA-EGFP
Tags8xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-Myr-PTEN
Plasmid#206983PurposeLentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signalDepositorInsertPTEN (PTEN Human)
UseLentiviralTagsN-terminal myristoylation siteExpressionMammalianPromotertet-on promoterAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
NG0886 pCI-TPI-WT-4H
Plasmid#65801PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
Tags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.U6.gRNAS1
Plasmid#58252PurposeU6 promoter-driven single guide RNA complementary to the human AAVS1 locusDepositorInsertU6.gRNAS1 cassette
UseAdenoviral and CRISPRExpressionMammalianAvailable SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-29
Plasmid#172753PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-29
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mNINJ1-D53A-GFP
Plasmid#208778PurposeMSCV vector expressing D53A mouse NINJ1DepositorInsertNINJ1 (Ninj1 Mouse)
UseRetroviralTagsGFPMutationChanged aspartic acid 53 to alanine (D53A)Available SinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY017 (pcDNA3.1-hPcCpf1)
Plasmid#69989PurposeExpresses humanized PcCpf1DepositorInserthPcCpf1
UseCRISPRTagsNLS & 3xHAExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-21
Plasmid#172749PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-21
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-37
Plasmid#172756PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-37
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant
Plasmid#87350PurposeS867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET constructDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/S355C/C574S/S867CPromoterT7Available SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOCC61
Plasmid#118877Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
TagsmGFP, HIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Nd1
Plasmid#191523PurposeExpression of mitochondrial v8(HS1) ZF-DdCBE pair in mammalian cells (LB510-Nd1+RB54-Nd1); AAV packaging vectorDepositorInsertv8(HS1) ZF-DdCBE pair LB510-Nd1+RB54-Nd1 AGKS
UseAAVExpressionMammalianPromoterCMVAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0013
Plasmid#117549PurposeLevel 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp15-DARPinOFF7
Plasmid#182399PurposeBacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusionDepositorInsertVNp15-DARPinOFF7
TagsVesicle Nucleating peptide 15 (VNp15)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT305_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PL-FRT5
Plasmid#52539PurposeExpression of FRT5-flanked Neo; homologous recombination targeting vector cloningDepositorInsertsPhiC31o attPx3 (70 bp each)
Neo
ExpressionMammalianAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pACEC3GH (Apr)
Plasmid#110085PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only