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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,598 results for: LEA

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Showing: 5161 - 5180 of 16598 results

  1. pAAV-hSyn-ChRger3-TS-EYFP

    Plasmid
    #127240
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger3-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Promoter
    hSyn1
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127240-plasmid-map-sequence-id-247091

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  9. pcDNA3-ABKAR

    Plasmid
    #140051
    Purpose
    Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.
    Depositor
    Jin Zhang
    Article
    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4
    Insert
    ABKAR
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    140051-plasmid-map-sequence-id-307466

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  12. pVSL10

    Plasmid
    #201182
    Purpose
    Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable
    Depositor
    Jack Szostak
    Article
    Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.
    Insert
    Bst polymerase large fragment (BF) F710Y/D598A
    Tags
    HRV 3C protease cleavage site and His6
    Expression
    Bacterial
    Mutation
    F710Y D598A
    Promoter
    T5-lacO
    Available Since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201182-plasmid-map-sequence-id-406202

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  18. MSP977

    Plasmid
    #65774
    Purpose
    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E mutation in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65774-plasmid-map-sequence-id-112656

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Showing: 5161 - 5180 of 16598 results