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Plasmid#158136PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
UseTagsExpressionYeastMutationCloned in a TAG codon at the third alanine residu…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG4
Plasmid#158137PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
UseTagsExpressionYeastMutationCloned in a TAG codon at the third serine residue…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFPJ1
Plasmid#83561PurposeExpress yEGFP-ERG9 ER signal peptide under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER signal peptide
UseTagsExpressionYeastMutationPromoterTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW022
Plasmid#83532Purposepheromone mediated expression of UBiC, ARO4, and TKL1DepositorInsertpFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t-pFUS1J2-TKL1-CYC1t
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW015
Plasmid#83530PurposeUBiC and feedback resistant ARO4 expression from pheromone inducible FUS1J2 promoterDepositorInsertpFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW018
Plasmid#83531PurposeAromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpinDepositorInsertpARO9-mf_2-pFUS1J2-ARO7i-CYC1t
UseTagsExpressionYeastMutationPromoterpARO9/pFUS1J2Available sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas59
Plasmid#82396PurposesgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas45
Plasmid#82390PurposesgRNA targeting YFP with additional bases 5' end like pCas34, but from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas57
Plasmid#82395PurposesgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas56
Plasmid#82394PurposesgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas55
Plasmid#82393PurposesgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas52
Plasmid#82392PurposesgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas39
Plasmid#82389PurposesgRNA targeting glnA expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas36
Plasmid#82388PurposesgRNA targeting ccmK expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas35
Plasmid#82387PurposesgRNA targeting cpcB expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas15
Plasmid#82383PurposesgRNA targeting YFP (truncated to 18 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas14
Plasmid#82382PurposesgRNA targeting YFP (truncated to 15 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas13
Plasmid#82381PurposesgRNA targeting YFP (truncated to 12 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMY01AB-PRO
Plasmid#127714PurposeIntegrative yeast promoter characterization plasmid. Clone in with BpiI. Has ChrXV homology upstream and an mVenus fragment downstream.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pEMY01CD-TER
Plasmid#127751PurposeIntegrative yeast terminator characterization plasmid. Clone in with BpiI. Has mVenus fragment upstream and a KanMX fragment downstream.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Plasmid EUPp_09
Plasmid#157745PurposePgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)DepositorInsertsUseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Cas9-2A-GFP-sgRNA
Plasmid#124889PurposeContains spCas9-2A-GFP and guide RNA scaffold in single retroviral vectorDepositorInsertCas9
UseRetroviralTags2A-GFPExpressionMutationPromoterMSCVAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase
Plasmid#87741PurposeIPTG-inducible expression of T4 DNA ligase for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHisExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]
Plasmid#227246PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1α and pTRE3GAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p-DsbC
Plasmid#202485PurposeExpresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutivelyDepositorInsertsulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)
UseTagsExpressionBacterialMutationPromoterEM7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-CmCHS-MsCHI
Plasmid#73394PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
CmCHS
MsCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas9cur
Plasmid#71538PurposeConstitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
Plasmid curing system
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-RLuc8
Plasmid#87121PurposeMammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.DepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationA55T, C124A, S130A, K136R, A143M, M185V, M253L, a…PromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]
Plasmid#227245PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1α and pTRE3GAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL
Plasmid#169533PurposeTier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).DepositorInsertPCMV-driven ER-localized monomeric red fluorescent protein mRuby2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only