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Plasmid#177351PurposeAAV expression of scFV-fused catalytic domain of TET1 for targeted DNA methylation editingDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseAAVTagsmyc and scFVExpressionMammalianMutationN terminal domain of human Tet1PromoterCMV promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP-alpha-Tubulin-19-C
Plasmid#108413PurposeAlpha-tubulin fused to monomeric near-infrared fluorescent protein miRFPDepositorInsertalpha-tubulin b (Tuba1b Mouse)
UseTagsmiRFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF0451
Plasmid#141616PurposeLentiviral vector for overexpressing the THRA transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTHRA (THRA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLH_Scr15
Plasmid#100537PurposeCEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCreC, CreN, and SV40 NLSExpressionYeastMutationN-terminal version of PhyB (AA 1-621)/ Mutation o…PromoterFBA1 and TDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-KLF6
Plasmid#192909PurposeBarcoded piggybac transposon vector with Dox-inducible expression of KLF6DepositorInsertKLF6 (KLF6 Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-IGFBPL1-Fc(DAPA)-AviTag-6xHis
Plasmid#156704PurposeMammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.DepositorInsertIGFBPL1 (IGFBPL1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EYS_WT
Plasmid#81854PurposeGateway Donor vector containing EYS ,part of the Target Accelerator Plasmid Collection.DepositorInsertEYS (EYS Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLH_Scr16
Plasmid#100538PurposeCEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCdc1 NLS, CreC, CreN, and SV40 NLSExpressionYeastMutationAA 1-621 of PhyB/ Mutation nucleotide 576 C-->APromoterFBA1 and TDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC32A1
Plasmid#132112PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC32A1 (SLC32A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
flag-hKLF6-4A (1010)
Plasmid#49489Purposeexpresses human Flag tagged KLF6 with 4A mutationDepositorInsertKFL6-4A (KLF6 Human)
UseTagsFlagExpressionMammalianMutationthree serines and one tyrosine mutated to alanin…PromoterCMVAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-CXXC/LLAA
Plasmid#124399PurposeFull-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)
UseTagsFlagExpressionMammalianMutationCXXC zinc finger deletion (aa 585-624) and L897A/…PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EYS_p.N269H
Plasmid#81557PurposeGateway Donor vector containing EYS ,part of the Target Accelerator Plasmid Collection.DepositorInsertEYS (EYS Human)
UseGateway entry vectorTagsExpressionMutationN269HPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-scFVtet1_bGHpA
Plasmid#177357PurposeAAV expression of scFV-fused catalytic domain of TET1 from doxycycine-inducible promoter for targeted DNA methylation editingDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseAAVTagsmyc and scFVExpressionMammalianMutationN terminal domain of human Tet1PromoterTetracycline-dependent promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0452
Plasmid#143879PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTHRA (THRA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0453
Plasmid#142628PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTHRA (THRA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EYS_p.F336L
Plasmid#81582PurposeGateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.DepositorInsertEYS (EYS Human)
UseGateway entry vectorTagsExpressionMutationF336LPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EYS_p.H466N
Plasmid#81517PurposeGateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.DepositorInsertEYS (EYS Human)
UseGateway entry vectorTagsExpressionMutationH466NPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EYS_p.R26Q
Plasmid#81523PurposeGateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.DepositorInsertEYS (EYS Human)
UseGateway entry vectorTagsExpressionMutationR26QPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EYS_p.R587I
Plasmid#81485PurposeGateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.DepositorInsertEYS (EYS Human)
UseGateway entry vectorTagsExpressionMutationR587IPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EYS_p.C554S
Plasmid#81491PurposeGateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.DepositorInsertEYS (EYS Human)
UseGateway entry vectorTagsExpressionMutationC554SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only