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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,850 results for: nin

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Showing: 501 - 520 of 66850 results

  1. TFORF3549

    Plasmid
    #145025
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145025-plasmid-map-sequence-id-419572

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  3. pCMV-MMLVgag-3xNES-Cas9

    Plasmid
    #181752
    Purpose
    MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.
    Depositor
    David Liu
    Publication
    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLV-Gag-3xNES-Cas9 (Spy)
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    181752-plasmid-map-sequence-id-353936

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  8. lentiCRISPR - EGFP sgRNA 1

    Plasmid
    #51760
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51760-plasmid-map-sequence-id-392672

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  9. pSL1142 (pSPIN, pBBR1 backbone)

    Plasmid
    #160730
    Purpose
    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Publication
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    160730-plasmid-map-sequence-id-357931

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  12. pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

    Plasmid
    #61628
    Purpose
    Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter
    Depositor
    Dolf Weijers
    Publication
    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:
    Inserts
    ntdTomato
    n2xEGFP
    Expression
    Plant
    Mutation
    2 copies of EGFP with N-terminal SV40 NLS
    Promoter
    Auxin-dependent DR5 promoter containing 9 reverse…
    Available since
    March 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61628-plasmid-map-sequence-id-396550

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Showing: 501 - 520 of 66850 results