pGG431
Plasmid
#165615
Purpose
Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)
Depositor
Insert
TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
Use
CRISPR
Tags
SV40 NLS
Expression
Yeast
Mutation
Corrected homology relative to wild type SpCas9 (…
Promoter
TEF1
Available Since
April 21, 2021
Availability
Academic Institutions and Nonprofits only
