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pRetro-CAG-DsRedExpress-TVA

Plasmid

#235699

Purpose

Control plasmid expressing TVA and DsRed without G protein

Depositor

Guo-li Ming, Hongjun Song

Article

Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

Insert

DsRedExpress, TVA800

Use

Retroviral

Promoter

CAG

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHJ3: LexA-VHH-Control

Plasmid

#108241

Purpose

LexA DNA binding domain fusing with anti-RNase A VHH antibody

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

LexA DNA binding domain fusing with anti-RNase A VHH antibody

Use

Synthetic Biology

Promoter

pLacO1

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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retro-gfpIkkb-puro vector

Plasmid

#58251

Purpose

Retroviral expression vector encoding bicistronic EGFP-IKKbeta and Puromycin cassette

Depositor

Georgios Stathopoulos

Article

Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

Insert

Ikkβ (Ikbkb Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pIG-828_HA-GD2-28z_CAR_BATF_Retroviral

Plasmid

#207505

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF, HA-GD2-28z_CAR (BATF Human)

Use

Retroviral

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAJE-Ma pylRS nitroY/haloY-F5

Plasmid

#225684

Purpose

Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.

Depositor

Ryan Mehl

Inserts

M. alvus PylRS 3-NY/HaloY F5
M. alvus Pyl-tRNA(6)

Use

Methanomethylophilus alvus

Mutation

CTG125CTT, N166A, V168S, A223C, W239R

Promoter

GlnS and Lpp

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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FUGW-H1-Scrambled control shRNA

Plasmid

#40625

Depositor

Sally Temple

Article

Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

Insert

Scrambled control shRNA

Use

Lentiviral and RNAi

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pLY096-RetroEFS-CD22BBz-WPRE

Plasmid

#192197

Purpose

Retro-CD22CAR

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-CD22CAR (CD22 Synthetic)

Use

Retroviral; Mammalian expression

Mutation

NA

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-831_HA-GD2-28z_CAR_TFAP4_Retroviral

Plasmid

#207508

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

Retroviral

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-flex-Voltron

Plasmid

#119035

Purpose

Cre-dependant expression of Voltron in neurons

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Use

AAV

Expression

Mammalian

Promoter

hsyn

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-IRSp53 S269D

Plasmid

#221319

Purpose

Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 (Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG

Mutation

S269D

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCFJ2259 - Intron GG2 - 900 bp

Plasmid

#159881

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - 900 bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE zeo Ecotropic Receptor

Plasmid

#10687

Depositor

William Hahn

Insert

Ecotropic Receptor (Slc7a1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pCFJ2215 - Intron GG3 - 900 bp

Plasmid

#159882

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG3 - 900 bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2214 - Intron GG1 - 900 bp

Plasmid

#159880

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - 900 bp

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-Control-PTEN-3'UTR-Wt

Plasmid

#21326

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

PTEN 3' UTR Wild Type (PTEN Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-flex-Positron-ST

Plasmid

#129267

Purpose

Cre-dependant expression of soma-localized Positron in neurons

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

AAV

Expression

Mammalian

Mutation

D92N, E199V

Promoter

hsyn

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight_A-CREB_PGK-tdTomato

Plasmid

#154264

Purpose

Dox/Tet-inducible expression of dominant negative CREB (A-CREB) in mammalian cells.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

dominant negative CREB

Expression

Mammalian

Promoter

Tight/tetO

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1 intron 1 gRNA as2

Plasmid

#132394

Purpose

Targets AAVS1 intron 1, gRNA: AGAACCAGAGCCACATTAAC, MLM3636 backbone

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

gRNA targeting AAVS1 intron 1 (PPP1R12C Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-no_intron

Plasmid

#127597

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

1xFLAG-MCP expression cassette

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-flex-Voltron-ST

Plasmid

#119036

Purpose

Cre-dependant expression of soma-localized Voltron in neurons

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Has Service

AAV1

Insert

Voltron-ST

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hsyn

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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