We narrowed to 17,077 results for: Por

1. pAD-GamS

   Plasmid

   #99246

   Purpose

   Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   gamS (with C-terminal 6xHis tag)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCXLE-dCas9VP192-T2A-EGFP-shP53

   Plasmid

   #69535

   Purpose

   Episomal plasmid encoding dCas9VP192 and p53 shRNA

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   dCas9-dCas9VP192-GFP-shp53
   

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-GRAB_r5-HT1.0

   Plasmid

   #208719

   Purpose

   Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Red fluorescent 5-HT sensor GRAB_r5-HT1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRPF215

   Plasmid

   #106377

   Purpose

   C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

   Depositor

   Neil Fairweather, Robert Fagan

   Article

   Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

   Insert

   Ptet-himar1-Ter(slpA)-transposon
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Codon optimised for C. difficile

   Promoter

   Ptet

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-h.Rhno1 S141A (C)-C-Myc-DDK-P2A-Puro

   Plasmid

   #211622

   Purpose

   Rhno1 S141A

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   hRHNO1 (RHNO1 Human)
   

   Use

   Tags

   myc-flag

   Expression

   Mammalian

   Mutation

   S141A

   Promoter

   CMV

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-PhoDer

   Plasmid

   #188473

   Purpose

   Expresses the optogenetic human Gasdermin D (PhoDer) in mammalian cells

   Depositor

   Gary Mo

   Article

   Santa Cruz Garcia et al Nat Commun. 2022 Jan 10;13(1):52. doi: 1

   Insert

   PhoDer (GSDMD Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available Since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSplit2-NO-PRPF6

   Plasmid

   #51740

   Purpose

   Express LucN-PRPF6 fusion protein in mammalian cells

   Depositor

   Hiroyoshi Ariga

   Article

   Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

   Insert

   PRP6 (PRPF6 Human)
   

   Use

   Tags

   FLAG and Luciferase (1-398aa)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCMV Puro DEST P38KTRDronpa

   Plasmid

   #205763

   Purpose

   Expression of P38 KTR Dronpa under CMV promoter (With Puromycin Resistance)

   Depositor

   Edward Boyden

   Article

   Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

   Insert

   P38 KTR Dronpa
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMPMA3dPlac-PpipB-GFPLVA

   Plasmid

   #23344

   Depositor

   Olivia Steele-Mortimer

   Article

   Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

   Insert

   GFP(LVA)
   

   Use

   Tags

   GFP(LVA)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. EpsilonCOP EGFP

    Plasmid

    #128151

    Purpose

    Expression of epsilon COP

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

    Insert

    epsilon COP (COPE Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTrcHIS-7D12Gln13TAG

    Plasmid

    #125264

    Purpose

    Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF

    Depositor

    Angus Johnston

    Article

    Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

    Insert

    Gln13TAG 7D12
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSC168

    Plasmid

    #216350

    Purpose

    xhNup35-Nb1t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup35-Nb1t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET264-pUC 24xMS2V6 Loxp KANr Loxp

    Plasmid

    #104393

    Purpose

    Yeast endogenous mRNA tagging

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUPD+Red-F

    Plasmid

    #170874

    Purpose

    Phytobrick - Red-F Luciferase CDS

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    Red-F
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    p.S286Y Red mutant

    Promoter

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FRB-VMAc-CSarcin

    Plasmid

    #70223

    Purpose

    Expresses VMA intein with C-terminal portion of alpha-sarcin

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (C-terminal portion)
    

    Use

    Tags

    FKBP-rapamycin binding (FRB) domain of the mammal…

    Expression

    Mammalian

    Mutation

    Changed Asp 28 to Cys, deleted amino acids 1-27

    Promoter

    CMV

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

16. NSarQ27G-VMAn-FKBP

    Plasmid

    #70230

    Purpose

    Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (N-terminal portion)
    

    Use

    Tags

    FK506- and rapamycin-binding protein (FKBP), Link…

    Expression

    Mammalian

    Mutation

    Changed Gln 27 to Gly

    Promoter

    CMV

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

17. AAV.hSyn.GLPLight1

    Plasmid

    #187466

    Purpose

    Expresses GLPLight1 in neurons

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

    Insert

    GLPLight1
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBEN276

    Plasmid

    #69150

    Purpose

    Integration of luciferase operon in Tn7 insertion site of Gram negative bacteria

    Depositor

    Pierre Germon

    Article

    Howe et al BMC Microbiol. 2010 Jul 23;10:197. doi:

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    frr

    Available Since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLD-puro-MTS-EGFP

    Plasmid

    #190270

    Purpose

    Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

    Depositor

    Christian Münch

    Article

    Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

    Insert

    MTS-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

    Plasmid

    #104394

    Purpose

    Yeast MCP-GFP expression

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    MCP-NLS-2xyeGFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    MCP fused to NLS(SV40) and 2x yeast codon optimiz…

    Promoter

    CYC1

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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